BmNPV的PK基因和杆状病毒PK基因的进化分析

PROTEIN KINASE GENE OF BmNPV AND THE PHYLOGENETIC TREE FOR BACULOVIRUS PROTEIN KINASE GENES

家蚕核型多角体病毒镇江株（BmNPVZJ8）蛋白激酶（BmvPK－1）基因编码区含828个核苷酸，可编码推测分子量为32kD的多肽。应用GCG程序分析表明BmvPK－1与AcMNPV的PK－1相似性最高，两者氨基酸序列相似性达95.2％。比较同时表明，BmvPK-1氨基酸序列与其它生物Ser／ThrPK的催化结构域有很高的同源性，与人的HSPKCE，果蝇的dPKC98F和酵母的TPK1的催化结构域分别有31．1％、32．3％和31.2％的相似性。在PK的进化树中，杆状病毒PK是一独立进化的分枝，BmvPK-1与PK－1进化距离最近，而HavPK则与HzSNPV的PK距离最近。

The coding region of BmvPK-1 gene of Bombyx mori nuclear polyhedrosis virus (BmNPV)Strain 2J8 is 828 nt long and potential to encode a polypeptide with predicted molecular mass of 32 kD. Analysis with GCG program shows BmvPK-1 is most closely related to PK-1 of AcMNPV,with 95.2% similarity in amino acid sequence. Comparison of the predicted amino acid sequence of BmvPK-1 with Ser/Thr PK of human,fruit fly and yeast also shows a high degree of homology,with similarities of 31. 1%, 32. 3 % and 31. 2%, respectively. A phylogenetic tree drawn by using PC/GENE program for PKs from 7 NPVs and 3 eukaryotic organism showed that 7 NPV PKs are in an independent branch, and there is the closest phylogenetic relationship between BmNPV BmvPK-1 and AcMNPV PK-1,and between HavPK and HzSNPV PK.