华蟾素注射液对大鼠血清促甲状腺素和肾上腺素含量的影响

Effects of cinobufacini injection on contents of serum thyroid-stimulating hormone and adrenaline in rats

目的:观察华蟾素注射液对大鼠血清促甲状腺素(thyroid-stimulating hormone,TSH)和肾上腺素(adrenaline,ADR)含量的影响,通过与寒证方和热证方对照,推导其寒热药性。方法:健康雄性Wistar大鼠32只随机分为空白对照组、寒证模型组、热证模型组和华蟾素组,每组8只。检测各组大鼠用药前、后体质量和血清TSH、ADR含量。结果:热证模型组大鼠用药后体质量低于空白对照组(P<0.05);华蟾素组、寒证模型组大鼠用药后血清TSH含量略低于空白对照组(P>0.05),热证模型组高于空白对照组(P>0.05);华蟾素组、寒证模型组大鼠用药后血清ADR含量低于空白对照组(P<0.05),热证模型组高于空白对照组(P<0.05)。结论:华蟾素可以抑制大鼠促甲状腺激素释放激素-促甲状腺激素-甲状腺激素系统和交感神经系统机能水平,与寒性药物作用特点相应,为推导华蟾素药性可能是寒性的结论提供了初步的实验依据。

Objective： To observe the effects of cinobufacini injection on serum levels of thyroid-stimulating hormone （TSH） and adrenaline （ADR） in rats, and to speculate the property （cold or heat） of the drug. Methods： Thirty-two male Wistar rats were randomly divided into normal control group, cinobufacini injection group, cold syndrome group and heat syndrome group. The rats in the four groups were administered with normal saline, cinobufacini injection, and compound of traditional Chinese drugs with heat or cold property. The weights, and the serum TSH and ADR levels of the rats were measured before and after the interventions. Results ： The weights of the rats in heat syndrome group markedly decreased after t4-day administration when compared with the normal control group （P〈0.05）. There were no significant differences among the normal control group, heat syndrome group and the cinobufacini injection group. Although no statistical difference was noted, the serum TSH levels of the cold syndrome group and cinobufacini injection group were slightly lower than that in the normal control group after 14-day administration, and the heat syndrome group showed a slightly higher serum TSH level. After administration, the serum ADR levels of the cold syndrome group and cinobufacini injection group were significantly lower than that in the normal control group （P〈0.05）, and the serum ADR level in the heat syndrom group was higher than that in the normal control group （P〈0.05）. Conclusion： Cinobufacini injection seemed to be able to suppress the thyrotrophin-releasing hormone-TSH- thyroxine system and sympathetic nervous system, matching up with the characteristics of the Chinese herbs of cold property. And the study provided initial experiment evidence for the conclusion that the property of cinobufacini might be cold.