醋酸铅在家兔体内的代谢动力学研究

Toxicokinetics Study of Lead Acetate in Domestic Rabbits

目的研究静注醋酸铅在家兔体内的代谢动力学模型。方法给家兔静注醋酸铅3mg/kg后,分别于第10、20、30、60、90、120、180、240、360min,用微分电位溶出法测定血液中的铅,用DAS2.0软件进行数据分析。结果微分电位溶出法测定血铅的线性范围为10～50μg/ml。醋酸铅主要代谢动力学参数分别为:中央室半衰期(t1/2α)为8.60min,浅外室半衰期(t1/2β)为67.69min,深外室半衰期(t1/2γ)为729.84min,中央室表观分布容积(V1)为77033.08L/kg,总清除率(CL)709.27L/(min·kg),0～t时的血药浓度-时间曲线下面积[AUC(0-t)]为3106.59ng/(L·min)。根据AIC(Akaike's information criterion)最小原则判断,在静脉注射3mg/kg醋酸铅后,其在家兔体内的代谢过程符合三室模型。结论静脉注射醋酸铅在家兔体内的代谢动力学符合三室模型,按一级速率过程消除。

Objective To research the toxicokinetics model of lead acetate in the domestic rabbits. Methods Injecting lead acetate into the vein of the domestic rabbit at the dose of 3 mg/kg, then the blood was collected at the 10th, 20th, 30th, 60th, 90th, 120th, 180th, 240th and 360th min. The concentrations of the blood lead were measured by differential potentiometrie stripping analysis, the data were analyzed by DAS2.0 software. Results The linear was at the range of 10 to 50 μg/ml, the major toxicokinetics parameters were： t1/2α = 8.60 min,t1/2β = 67.69 min,t1/2γ = 729.84 min, V1=77 033.08 L/kg, CL = 709.27 L/（min·kg）,AUC（0-t） = 3 106.59 ng/（L ·min）. According to the smallest principle of AIC, the lead concentration in vivo conformed to the three compartmental models in domestic rabbit after injecting lead acetate at 3 mg/kg. Conclusion The lead concentration conforms to the three compartmental models in the domestic rabbit after intravascular injection of lead acetate, and it is eliminated according to the first order processes.