北京早园竹叶提取物的抗氧化活性研究

Anti-oxidative Activity of Extract of North Ph. Praecox Leaves

目的初步研究北京早园竹叶提取物的抗氧化能力。方法采用超声辅助法提取竹叶提取物,NaNO3-Al(NO3)3-NaOH显色法检测总黄酮浓度,并通过二苯基苦基苯肼(DPPH)自由基清除法和CuSO4-Phen-Vc-H2O2-DNA体系化学发光法对其进行抗氧化能力测定。结果提取物清除DPPH自由基的IC50为7.02mg/L;竹叶提取物可延迟DNA损伤峰的出峰时间(P<0.01),浓度大于400mg/L的竹叶提取物可防止DNA氧化损伤。结论竹叶提取物具有一定的DPPH自由基清除能力,浓度大于400mg/L的竹叶提取物可显著抑制DNA损伤。

Objective To study the antioxidant activity of the extract from north Ph.praecox leaves. Methods The extract from north Ph.Praecox leaves was made by ultrasonic-assisted extraction process, and the concentration of the total flavonoids was detected by NaNO3-Al（NO3）3-NaOH methods. The antioxidant capacity of the extraction was detected by DPPH free radical scavenging method and CuSO4-Phen-Vc-H2O2-DNA reaction with ultra-weak chemiluminescence method. Results The bamboo leaf extract could scavenges the DPPH free radical with the IC50 value as 7.02 mg/L and could delay the development of the emission peak value （P〈0.01 ）, the extract could also prevent the occur of the peak value of the emission when the concentration of it is higher then 400 mg/L. Conclusion The extract from north Ph.praecox leaves has the capacity of scavenging DPPH free radical , the extract of bamboo leaves could protect DNA from damaged significantly when the concentration is higher then 400 mg/L.