低能N^＋注入对小麦反转录转座子WIS2-1A表达活性的影响

Influence of low-energy N^+ implantation on the transcription activity of retrotransposon WIS2-1A in wheat

本实验采用不同剂量低能N^+注入小麦种子,研究种胚在萌动的不同阶段反转录转座子WIS2-1A的表达活动,分析低能N^+注入对WIS2-1A表达的时间效应和剂量效应。结果显示种子培养初始的60 h内,室温对照组、真空对照组、0.5×10^(17) N^+/cm^2注入组和1.0×10^(17) N^+/cm^2注入组的种胚中,反转录转座子WIS2-1A始终维持低水平的表达,且随着种子培养时间延长,表达活性显著下降。经过0.5×10^(17)N^+/cm^2处理的小麦,在培养30 h时,种胚内WIS2-1A的相对表达量最高,达到对照的138倍。该发现表明反转录转座子WIS2-1A可能在低能离子束对当代小麦的诱变效应中起一定作用。掌握WIS2-1A对不同剂量低能N^+注入的敏感性和它的表达活动规律,能够为低能离子注入生物有机体产生诱变效应的理论研究提供基础。

n this work, we studied the transcription activity of retrotransposon WIS2-1A responding to different doses of N^＋ implantation at different growth stages of wheat seeds. The time and dose effect of N^＋ implantation on the transcription of WIS2-1A were analyzed. The results showed that the transcription activity of WIS2-1A decreased with the prolonged growth time in the first 60 h of incubation in the room temperature and vacuum control groups, and the groups implanted with 0.5×10^17 N^＋/cm^-2 and 1.0×10^17 N^＋/cm^-2. The relative quantification of transcripts in the group implanted with 0.5×10^17 N^＋/cm^-2 and incubated for 30 h was 138 times higher than the control. This indicated that the retrotransposon WIS2-1A probably attributed to the mutation effect of the present generation of wheat. The sensitivity and transcription activity of retrotransposon WIS2-1A responding to N^＋ implantation could ground the theory of mutation effect induced by low-energy ions implantation.