JS-K对Hela细胞、A375细胞和HL-60细胞增殖的影响

Effect of JS-K on the cell proliferation in human HL-60,A375 and Hela cells

目的探讨JS-K对人白血病HL-60细胞、黑色素瘤A375细胞以及宫颈癌Hela细胞增殖的影响。方法采用MTT法检测细胞生长情况,观察JS-K在0.3～10.0μmol/L浓度范围内对Hela细胞、A375细胞和HL-60细胞生长的影响,计算其抑制率;在倒置显微镜下观察细胞的形态学变化。结果MTT法检测发现,JS-K在0.3～10.0μmol/L处理HL-60细胞24-96h,反映活细胞数量的OD值呈时间依赖性和剂量依赖性的降低;JS-K对HL-60细胞的生长的抑制率则明显增高;显微镜下也可见,3.0和10.0μmol/L的JS-K作用96h后,HL-60细胞的数量明显减少,细胞碎片增加;96hJS-K对于HL-60的IC50为0.58±0.01μmol/L。与空白组比较,0.3～10.0μmol/LJS-K作用Hela细胞、A375细胞24-96h,反映活细胞数量的OD值和镜下所见细胞数量和形态未见明显改变。结论JS-K对Hela细胞、A375细胞的增殖则无明显作用,而对HL-60细胞增殖具有明显的抑制作用,其作用机制值得进一步深入研究。

Objective To explore the effects of JS-K on the cells proliferation in human cervical carcinoma Hela cells, human melanoma A375 cells and human myeloid leukemia HL-60 cells. Methods The cell viability was detected by MTT method. The effect of JS-K （0.3-10.0 μmol/L） was observed on the growth of Hela ceils, A375 cells and HL-60 cells from 24 to 96 hours.Then the inhibition percentages of the growth of HL-60 cells proliferation were calculated. The morphologic changes were observed through inverted microscope. Results JS-K （0.3-10.0μmol/L） obviously decreased OD values of HL-60 cells by MTT method in a time-dependent and dose-dependent manner; And the inhibitory percentages of HL-60 cells growth were increased, The 50% inhibition concentration was 0.58±0.01μmol/L at 96 hour. JS-K did not affect on the OD value, and has no change of number and morphous in Hela cells and A375 cell. Condusions JS-K has inhibitory growth in HL-60 cells in vitro, but not in Hela cells and A375 cells. The anticancer mechanism is further worth of studying.