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Zebrafish:A Renewed Model System For Functional Genomics

Zebrafish:A Renewed Model System For Functional Genomics
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摘要 In the post genome era, a major goal in molecular biology is to determine the function of the many thousands of genes present in the vertebrate genome. The zebrafish ( Danio redo) provides an almost ideal genetic model to identify the biological roles of these novel genes, in part because their embryos are transparent and develop rapidly, The zebrafish has many advantages over mouse for genome-wide mutagenesis studies, allowing for easier, cheaper and faster functional characterization of novel genes in the vertebrate genome. Many molecular research tools such as chemical mutagenesis, transgenesis , gene trapping, gene knockdown, 在柱子染色体时代,在分子的生物学的一个主要目标是决定在脊椎动物染色体在场的许多几千基因的功能。zebrafish (Danio rerio ) 提供一个几乎理想的基因模型识别这些新奇基因的生物角色,部分地因为他们的胚胎是透明的并且很快发展。zebrafish 为染色体宽的 mutagenesis 研究比老鼠有许多优点,允许在脊椎动物染色体的新奇基因的更容易、更便宜、更快的功能的描述。许多分子的研究工具象化学 mutagenesis 那样, transgenesis,套住的基因,基因击倒, TILLING,指向的基因, RNAi 和化学基因屏幕现在在 zebrafish 是可得到的。联合所有前面、反向、化学的基因工具, zebrafish 将做无价的贡献到脊椎动物,这被期望在基因,当模特儿的人的疾病和药发现的功能的注解的功能的 genomics。
作者 Xiao-Yan Wen
出处 《Science Foundation in China》 CAS 2008年第2期36-46,共11页 中国科学基金(英文版)
关键词 Instrumentation and measurement Medical physics Biological physics 功能基因组学 斑马鱼 模型系统 后基因组时代 脊椎动物 分子生物学 遗传模型 生物作用
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参考文献13

  • 1Jianguo Su,Zuoyan Zhu,Yaping Wang,Feng Xiong,Jun Zou.The Cytomegalovirus Promoter-Driven Short Hairpin RNA Constructs Mediate Effective RNA Interference in Zebrafish In Vivo[J].Marine Biotechnology.2008(3)
  • 2Wei-yi Liu,Yun Wang,Yao Qin,Ya-ping Wang,Zuo-yan Zhu.Site-Directed Gene Integration in Transgenic Zebrafish Mediated by Cre Recombinase Using a Combination of Mutant Lox Sites[J].Marine Biotechnology.2007(4)
  • 3Xiufang Pan,Haiyan Wan,Wendy Chia,Yan Tong,Zhiyuan Gong.Demonstration of site-directed recombination in transgenic zebrafish using the Cre/loxP system[J].Transgenic Research.2005(2)
  • 4Stanford WL,Caruana G,Vallis KA,Inamdar M,Hidaka M,Bautch VL,Bernstein A.Expression trapping:identification of novel genes expressed in hematopoietic and endothelial lineages by gene trapping in ES cells[].Blood.1998
  • 5Summerton J.Morpholino antisense oligomers:the case for an RNase H-independent structural type[].Biochimica et Biophysica Acta.1999
  • 6McGraw HF,Nechiporuk A,Raible DW.Zebrafish dorsal root ganglia neural precursor cells adopt a glial fate in the absence of neurogeninl[].The Journal of Neuroscience.2008
  • 7Delalande JM,Guyote ME,Smith CM,Shepherd IT.Zebrafish sipla and siplb are essential for normal axial and neural patterning[].Developmental Dynamics.2008
  • 8Su J,Zhu Z,Wang Y,Xiong F,Zou J.The cytomegalovirus promoter-driven short hairpin RNA constructs mediate effective RNA interference in zebrafish in vivo[].Marine Biotechnology.2008
  • 9Doyon Y,McCammon JM,Miller JC,Faraji F,Ngo C,Katibah GE,Amora R,Hocking TD,Zhang L,Rebar EJ,Gregory PD,Urnov FD,Amacher SL.Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases[].Nature Biotechnology.2008
  • 10Stern HM,Murphey RD,Shepard JL,Amatruda JF,Straub CT,Pfaff KL,Weber G,Tallarico JA,King RW,Zon LI.Small molecules that delay S phase suppress a zebrafish bmyb mutant[].Nat Chem Biol.2005

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