原发性肝癌基质金属蛋白酶-9基因表达的研究

Matrix metalloproteinase-9 gene expression in patients with primary hepatocellular carcinoma

目的了解原发性肝癌患者基质金属蛋白酶-9（MMP-9）基因表达的情况。方法：33例原发性肝细胞癌患者术中取癌组织及癌旁组织；提取组织总KNA；用逆转录共扩增定量PCR方法测定基质金属蛋白酶-9（MMP-9）基因表迭水平。结果：肝癌组织基质金属蛋白酶-9基因表达水平明显高于癌旁的肝硬化组织及正常肝组织。肝癌患者基质金属蛋白酶-9基因表达水平与血清AFP浓度、肿瘤大小无关。结论：基质金属蛋白酶-9在原发性肝癌的进展中可能起重要作用。

Background and purpose： To investigate matrix metalloproteinase-9（MMP-9） gene expression in primary hepatocellular carcinoma. Methods： 33 patients with primary hepatocellular carcinoma were selected and cancerous and paracancerous tissue were obtaines during operation. Total RNA was isolated and matrix metalloproteinase-9 （MMP-9）gene expression level were measured by reverse transcriptionpolymerase chain reaction.Results： The matrix metalloproteinase-9 （MMP-9）gene expression level in cancerous tissue was much higher than those of cirrhotic tissue and normal liver tissue. MMP-9 gene expression levels has no correlation with serum concentration of AFP and tumor size. Conclusion; Matrix metalloproteinase-gmay play an important role in the progression of primary hepatocc!lular carcinoma.