重组人血管内皮抑素联合放疗对人类肺癌A549细胞株生长抑制及凋亡的影响

Effect of combining endostatin and radiation on growth inhibiting and apoptosis of A549 cell line

目的研究重组人血管内皮抑素（ES，商品名：恩度）联合放射治疗对肺腺癌A549细胞的生长抑制以及血管内皮生长因子（VEGF）分泌水平和细胞凋亡的影响。方法将培养至对数生长期的A549细胞分为空白对照组、单纯放疗组、单用ES组、联合治疗组，其中在联合治疗组中根据使用放疗和内皮抑素处理的时序不同又分为先ES后放疗组（ES—RT）、先放疗后ES组（RT—ES）、ES与放疗同时进行组（RT＋ES）。放疗处理时给予^60Co照射，2Gy，单次照射；ES处理时在细胞培养液中给予ES10ul。ELISA法测定ES联合放疗对肺癌A549细胞株VEGF分泌的影响并绘制细胞生存曲线，Hoechst染色观察细胞凋亡。结果ES和放疗均对肺癌A549细胞有生长抑制作用，但联合治疗组的抑制作用较其他各组更加明显且差异性具有统计学意义（P〈0．05）。处理后的A549细胞VEGF表达量在ES组、联合治疗的各亚组均较空白对照组明显减少（P〈0．05）。ES组、RT组、联合治疗的各亚组的凋亡率均较空白对照组明显升高（P〈0．05）。结论ES联合放疗与单用ES和单纯放疗相比较，前者在抑制肿瘤细胞生长以及促进A549细胞的凋亡方面效果更好。使用ES下调肺癌A549细胞VEGF的表达，具有增加放射治疗敏感性的作用。ES与放疗联合时序的不同，对放疗增敏作用的影响无明显区别。

Objective To investigate antiproliferative activity of endostatin（ES）, the combining effect of Endostar and radiation on VEGF and apoptosis of the A549 human lung adenocarcinoma cell line. Methods The cell of exponential phase of growth were divided into the control, radiation alone, ES alone, radiation after ES（ES---~RT）, ES after radiation（RT-ES）, radiation and ES at equal pace （RT＋ES）, T-ray radiation at a dose of 2 Gy, single fracination irradiation, YH-1610ug/ml in the cell culture bottle. The combining effect was quantified by the survival curve. ELISA Was used to observe the VEGF of radiation with ES on A549 cell lines. Apoptosis was observed by Hoechst staining. Results There showed that both of ES and combining treatment had the function of antiproliferative. But the union treatment＇s function was more obvious （P 〈0.05）. The expression of VEGF in ES and the combining treatment group was more lower than others （P 〈0.05）. On apoptosis, the rate of apoptosis in ES, RT, combining treatment group was higher than others （P 〈0.05）. Conclusion On antiproliferative activity and rate of apoptosis, the combining of ES and radiation is better than to use them alone. To reduce VEGF of the AS49 human lung adenocarcinoma cell line by ES could enhance radioresponse. There is no evidences to show that the expression of VEGF is concerned with the different choronological order of radiation and ES.