沙利度胺、5-氟尿嘧啶对胃癌细胞生长和凋亡作用的影响

Effect of thalidomide and 5-Fluorouracil on MGC-803 cell

目的探讨沙利度胺（Thai）、5-氟尿嘧啶（5-Fu）单用和联合应用对MGC-803细胞株的抑制增生、诱导凋亡的作用。方法采用AO／EB染色荧光显微镜观察处理细胞的形态变化，MTT法检测对细胞的增生抑制作用，流式细胞术（FAM）定量检测对细胞的诱导凋亡作用。结果在MTT分析中，单药Thal药物浓度≥25mg／L、5-Fu单药各浓度组与空白对照组相比均可增加对MGC-803细胞的增生抑制率（P〈0．05）。在5-Fu剂量为25mg／L时，联合用药组的增生抑制率增加更显著（P〈0．01），但两个联合用药组之间差异无统计学意义（P〉0．05）；在5-Fu剂量均为12．5mg／L时，联合使用沙利度胺50mg／L或100mg／L时与5-Fu25mg／L单药组相比差异均无统计学意义（P〉0．05）。而在FAM检测中，各实验组与空白对照组相比及各实验组两两比较，凋亡率差异均有统计学意义（P〈0．01）。结论Thai和5-Fu单用时对MGC-803细胞株均有抑制增生和诱导凋亡作用，联合应用时其作用明显增强，联合使用沙利度胺时可降低5-Fu的使用剂量。

Objective To investigate the proliferation inhibition and apoptosis induction effect of thalidomide（Thai） and its joint effect with 5-Fu on MGC-803 cell line. Methods The morphological changes of MGC-803 ceils with AO/EB stain were observed under fluorescence microscope. The proliferation inhibition effect was evaluated with MTT. The apoptosis induction effect was determined by FAM. Results The results of MTT array were as below： the difference was significant between Thal groups of 25 mg/L or above concentration and the control group （P 〈0.05）, 5-Fu of all testing concentrations showed significant difference compared with the control group（P 〈0.01）. For combined groups with the same concentration of 25 mg/L 5-Fu, combined groups showed distinct difference from the corresponding 5-Fu group （P 〈0.01）, but two combined groups showed no distinct difference with-each other （P〉0.05）. For combined groups with the same concentration of 5-Fu 12.5 mg/L, 5-Fu plus 50 mg/L or 100 mg/L Thal showed no distinct difference from 5-Fu group of 25 mg/L（P 〉0.05）. With FAM study, all test groups showed significant difference compared with the control group （P 〈 0.01）. There was also significant difference between two test groups arbitrarily （P 〈0.01）. Conclusion Both Thai and 5-Fu could inhibit the proliferation and induce the apoptosis of MGC-803 cells. The effect is reinforced with the combination significantly. Combination with Thal could decrease the concentration of 5-Fu but have the similar effect.