实时定量PCR研究大鼠肌肉组织基因表达

mRNA expression of rat muscle tissues by real-time quantitative PCR

运用实时定量聚合酶链式反应(RQ-PCR)研究了正常以及患有糖尿病大鼠的肌肉组织中胰岛素信号转导通路中重要的信号传导分子基因表达,结果表明:四氧嘧啶诱导的糖尿病大鼠肌肉组织的胰岛素受体、胰岛素受体底物1和葡萄糖转运体4的mRNA表达下调,胰岛素磷脂酰肌醇3激酶的mRNA表达上调;糖尿病大鼠骨骼肌葡萄糖转运体4的mRNA含量下降可能会引起糖摄取能力的下降,从而导致四氧嘧啶诱导大鼠肌肉组织胰岛素抵抗.

The mRNA expression levels of important insulin signal molecules in rat muscle of alloxan- induced diabetes were studied by real-time quantitative polymerase chain reaction （RQ-PCR）. The results demonstrated that the mRNA expression levels of IR, IRS1, and GI.UT4 in diabetic rat muscle tissue were up regulated, and the mRNA expression level of PI（3）K was down regulated. The de- crease in mRNA expression level of GLUT4 in diabetic rat muscle tissue implied that there might be insulin resistance in insulin signal system due to the decrease in its ability of glucose uptake.