大豆乳清废水发酵生产单细胞蛋白的酵母

Yeasts for single-cell protein production by soybean whey fermentation

为回收大豆乳清废水中的生物质资源,以白地霉(G.candidum link)、产朊假丝酵母(C.utilis)、热带假丝酵母(C.tropicalis)、解脂假丝酵母解脂变种(C.lipolytica var.lipolytica)和扣囊复膜孢酵母(S.fibuligera)5种常见的工业酵母菌,通过摇瓶发酵,进行大豆乳清废水的单细胞蛋白(SCP)生产试验研究.结果表明,被试5种酵母菌均能在大豆乳清废水中快速增殖,适宜的接种量为0.3～0.4 g/L;在废水COD质量浓度11150 mg/L、初始pH6.2、25℃、180 r/min等条件下发酵12 h,C.lipolytica var.lipolytica的细胞产量最高,可达2.35 g/L,对废水COD的去除率可达48.3%;在对数期生长期,S.fibuligera的细胞增殖速率、收获SCP的蛋白质含量和蛋白质产量分别为0.36 g/(L.h)、39.7%和0.88 g/L,居于被试5种酵母之首.C.lipolytica var.lipolytica和S.fibuligera是处理大豆乳清废水并回收SCP的最适酵母.

In order to reuse the nature biomass and remove some COD in soybean whey, Geotrichum candidum link, Candida utilis, Candida tropicalis, Candida lipolytica vat. lipolytica and Saccharomycopsis fibuligera were investigated for single - cell protein （SCP） production from soybean whey by batch fermentation. The results indicated that all the five yeasts could satisfactorily proliferate with an inoculant in the range from 0. 3 to 0. 4 mg/L. Among these five yeasts, C. lipolytica vat. lipolytica and S. fibuligera were proved to be the feasible yeasts for SCP production from soybean whey. When the initial COD concentration was 111500 mg/L and the pH was 6.2, the SCP of C. lipolytica var. lipolytica reached the maximum of 2.35 g/L with a COD removal of 48.3% after being cultured in Erlenmeyer flask for 12 h at 25 ℃ with rocking at 180 r/min. For S. fibuligera, the SCP specific production rate during logarithmic phase, the protein content and the protein yield were 0.36 g/（ L · h）, 39.7% and 0.88 g/L, respectively, which were the maximum among the five yeasts.