深低温保存与环氧氯丙烷干预脐动脉预作为同种血管材料的实验研究

Experimental study of umbilical artery treated with cyropreservation and epoxy chloropropane as homograft vessel

目的:研究液氮深低温保存与环氧氯丙烷(EC)联合处理新生儿脐动脉后的相关性能变化,探讨脐动脉作为小口径同种异体血管替代材料的可行性。方法:采用液氮深低温保存脐动脉,并用EC进行抗钙化处理;检测处理后血管的形态结构、物理性能改变,去抗原性和抗钙化效果。结果:液氮深低温保存60d内+EC处理后的脐动脉具有低免疫原性和低钙化性,有良好的耐压性能与顺应性。结论:采用液氮深低温+EC处理脐动脉的去抗原、抗钙化效果良好,血管物理性能变化小,血管可较长时间保存。具有作为小口径同种异体血管替代材料的可行性。

Objective：To study the changes of umbilical artery＇s （UA） concerned performance after treated with liquid nitrogen and epoxy ehloropropane （EC）, discuss the feasibility of UA as small diameter homograft substitute vessel. Method：UA was de-antigened by liquid nitrogen and anti-calcified by EC, after that the vascular morphological change, mechanical performance and the effect of de-antigenicity and anti-calcification were examined. Result： UA being cyropreserved by liquid nitrogen in 60 days and fixed by EC has lower antigenieity and lower calcifying capability, high pressure-endure capacity and favorable compliance. Conclusion： There are good deantigenicity and anti-calcification effects when UA being cyropreserved by liquid nitrogen and fixed by EC. The mechanical performance of the new UA does not change significantly, and it can be preserved in a longer time. The study concluded primarily that UA treated by our way has the feasibility as small diameter homograft substitute vessel.