水稻类钙调磷酸酶B亚基蛋白基因OsCBL8功能的初步研究

Preliminary Study on the Function of Calcineurin B-Like Protein Gene OsCBL8 in Rice

以转正义或反义OsCBL8基因的T3代株系及非转基因对照为材料,研究了转OsCBL8基因对水稻农艺性状、内源OsCBL8基因表达和幼苗耐盐、耐旱及耐冷性的影响。结果表明,供试的16个转基因株系的株高、单株分蘖数、单株有效穗数、穗长和百粒重等性状与对照无显著差异,每穗实粒数和结实率则大都显著或极显著低于对照。部分转基因株系的半定量RT-PCR结果表明,转正义基因显著增强了植株中OsCBL8基因的表达,而转反义基因则对内源OsCBL8基因的表达具有一定的抑制作用。在盐、干旱和低温等非生物胁迫条件下的生长实验发现,1个转正义基因株系的耐盐性得到显著增强,1个转反义基因株系的耐旱性得到显著增强。讨论了OsCBL8基因在水稻耐非生物逆境方面可能的机理。

The homozygous T3 transgenic lines with sense OsCBL8 and anti-sense OsCI3L8 obtained by Agro-transformation were used to investigate the function of OsCBL8 in rice. The results showed that either over-expression or inhibition of OsCBL8 gene in transgenic lines had little effect on main agronomic traits, but significantly decreased the number of filled grains per panicle and seed setting rate in some lines. Semi-quantitative RT-PCR showed that the expression of OsCBL8 extremely increased in sense transgenic seedlings, and decreased to some extent in anti-sense transgenic ones. By evaluation of tolerance to 150 mmol/L NaCl, 20 % PEG6000 and low temperature treatments, one sense transgenic line with high salt tolerance and one antisense transgenic line with high drought tolerance were obtained, respectively. It is discussed that the OsCBL8 gene may play an important role in rice tolerance to abiotie stresses.