生地预处理对全脑缺血再灌注大鼠血清TNF与NSE表达的影响

An experimental study on the shendi preconditioning effects on the expression of TNF and NSE in rats with cerebral ischemic-refusion injury

目的:观察比较中药生地预处理与缺血预处理减轻全脑缺血再灌注大鼠肿瘤坏死因子(TNF)与特异性神经元烯醇化酶(NSE)表达变化。方法:实验选用25只雄性SD大鼠,体质量180—220g,随机将25只大鼠分为5组(假手术组、缺血预处理组、生地预处理组、阿司匹林预处理组、脑缺血再灌注组),采用热凝椎动脉,钳夹双侧颈总动脉建立全脑缺血模型,缺血预处理组预缺血3min,3d后给予缺血10min,再灌注24h后处死。假手术组暴露双侧颈总动脉不夹闭,缺血再灌注组,夹闭双侧大脑颈总动脉10min,再灌注24h后处死。采用ELISA检测血清中TNF与NSE含量。结果:脑缺血再灌注后血清中TNF与NSE含量增加(P<0.05),生地预处理组与缺血预处理组血清中TNF与NSE含量降低,生地预处理组TNF与NSE含量与缺血预处理组比较P>0.05,两者与缺血再灌注组比较P<0.05。结论:生地预处理对随后的脑梗死有明显的保护作用,能诱导缺血耐受的产生。其机制可能是调节TNF与NSE的病理性表达。

Objective： To investigate the effects of shengdi preconditioning and cerebral ischemic preconditioning On the expression of tumor necrosis factor （TNF） and neuronal specific enolase （NSE） in the blood of rats with cerebral ischemia-reparfusion injury. Method： Twenty-five male SD rats （weight 180--220g） were randomly divided into 5 groups ： sham operation group （n=5） ：ischemic preconditioning group （n=5） ; shendi preconditioning group （n=5） ; aspirin （ASP） preconditioning group （n=5）;cerebral ischemia and reperfusion group（n=5）.The global cerebral ischemia and reperfusion model was established with Pulsinelli＇s method.The expressions of TNF and NSE were studied respectively in each group. Result： The expressions of TNF-α and NSE were very low in sham operation group. After isehemia-reperfusion, the expressions of TNF and NSE increased significantly（P〈0.05）. The contents of TNF and NSE in shendi preconditioning group and ischemic precondioning group all decreased（P〈0.05）. Conclusion： Shengdi preconditioning might provide obvious protection effect to the following cerebral is chemia and induced brain tissue tolerance to secondary severe ischemia through regulating the pathological expressions of TNF and NSE.