针刺不同穴位对脑缺血模型大鼠脑血流量的影响

Effects of acupuncture at different acupoints on cerebral blood flow in cerebral ischemia model rats

目的:探讨治疗缺血性脑梗死的有效方法及针刺治疗的穴位特异性。方法:Wistar成年雄性大鼠120只,随机分为正常组、假手术组、模型对照组、非针刺组和针刺组,针刺组又随机分为非穴组和"醒脑开窍"针刺法各主穴组,即水沟组、内关组、尺泽组、三阴交组和委中组。每组12只大鼠。复制大鼠大脑中动脉缺血模型(MCAO),分别对"醒脑开窍"针刺法主穴("水沟""内关""尺泽""三阴交""委中")以及非穴区,施以频率3次/秒、持续时间5s的针刺干预,以脑血流量为评价效应指标。结果:与模型对照组比较,非针刺组脑血流量有所升高,但差异无统计学意义(P>0.05);与非针刺组比较,针刺后的所有组别均可提高MCAO大鼠脑血流量(均P<0.05);与非穴组比较,所有穴位组的脑血流量均升高,水沟组和内关组升高明显(均P<0.05),而尺泽组、三阴交组、委中组与非穴组比较,差异无统计学意义(均P>0.05)。结论:①MCAO大鼠于梗死后72h内在脑血流方面存在自我修复和向愈的趋势;②给予针刺刺激后可促进MCAO大鼠脑血流量的改善,且改善作用明显高于其自身修复能力,是治疗缺血性脑卒中的有效方法;③"醒脑开窍"针刺法各主穴中,"水沟"和"内关"在改善MCAO大鼠脑血流量方面效果显著,有穴位特异性作用。

Objective To probe into effective methods for treatment of ischemic cerebral infarction and specificity of acupoints in the acupuncture treatment. Methods The rat model of middle cerebral artery occlusion （MCAO） was established with thread ligation according to Zea-Longa’s method. They were divided into normal group, non-operation group, model control group, non-acupuncture group and acupuncture group, and the acupuncture group was divided into sham-acupuncture group and four Xingnao Kaiqiao groups （contain Shuigou group, Neiguan group, Chize group, Sanyinjiao group and Weizhong group）. Then they were treated by acupuncture at ＂Shuigou＂ （GV 26）, ＂Neiguan＂ （PC 6）, ＂Chize＂ （LU 5）, ＂Sanyinjiao＂ （SP 6）, ＂Weizhong＂ （BL 40） and non-acupoints, 3 times/second, for 5 seconds. Cerebral blood blow （CBF） was used for assessment of the effect. Results As compared with the model control group, in the non-acupuncture group CBF did not significantly change （P〉0.05）; compared with the non-acupuncture group, after acupuncture CBF was significantly increased in the Shuigou group and the Neiguan groups （all P〈0.05）, but did not significantly increased in the Chize group, Sanyinjiao group and Weizhong group （all P〉0.05）.Conclusion CBF has a tendency of spontaneous cure within 72 h after cerebral infarction in the MCAO rat; acupuncture can significantly improve CBF in the MCAO rat, so it is an effective method for treatment of ischemic stroke; among the acupoints in the ＂Xingnao Kaiqiao＂ needling method, ＂Shuigou＂ （GV 26） and ＂Neiguan＂ （PC 6） have obvious effect in improvement of CBF, indicating acupoint specificity of ＂Shuigou＂ （GV 26） and ＂Neiguan＂ （PC 6） in treatment of ischemic stroke.