“化瘀贴”穴位贴敷对局灶性脑缺血大鼠VEGF表达的影响

Effect of acupoint sticking of "Huayutie" on VEGF expression in rats of focal cerebral ischemia

目的:探讨"化瘀贴"穴位贴敷对缺血性脑卒中的作用机制。方法:将80只Wistar大鼠随机分为模型组、穴位贴敷组、针刺组、尼莫地平组、正常组5组。采用电凝阻断大脑中动脉法制备大鼠局灶性脑缺血模型。模型组:造模后不做任何处理;穴位贴敷组:造模后采用"化瘀贴"贴敷"大椎""气海""命门"穴;针刺组:造模后针刺与穴位贴敷组相同穴位;尼莫地平组:造模后采用尼莫地平混悬液灌胃给药;正常组:不做任何处理。观察各组大鼠梗死灶体积、脑组织含水量、血管内皮生长因子(vascular endothelial growth factor,VEGF)表达和蛋白水平的变化。结果:梗死灶体积与电凝的大脑中动脉分支支配的区域吻合。各治疗组脑含水量与模型组比较,差异均有统计学意义(均P<0.05)。穴位贴敷组梗死灶周围VEGF阳性细胞数量及蛋白水平均较模型组增强(P<0.01),与尼莫地平组、针刺组比较差异无统计学意义(均P>0.05)。结论:"化瘀贴"穴位贴敷可能通过增加VEGF表达和蛋白水平减轻缺血后脑损伤。

Objective To explore the mechanism of acupoint sticking of ＂Huayutie＂ in improving ischemic stroke. Methods Eighty rats were randomly divided into 5 groups, a model group, an acupoint sticking group, an acupuncture group, a Nimodipine group and a normal group. Middle cerebral artery occlusion （MCAO） was used for preparation of focal cerebral ischemic rat model. After modeling, any treatment was not given to the model group; for the acupoint sticking group, ＂Huayutie＂ was applied at ＂Dazhui＂ （GV 14）,＂Qihai＂ （CV 6） and ＂Mingmen＂ （GV 4）; for the acupuncture group, acupuncture was given at the same acupoints as those in the acupoint sticking group; the Nimodipine group received intragastric administration of Nimodipine. And the normal group did not receive any treatment. Their infarction volume, the cerebral water content, expression of vascular endothelial growth factor （VEGF） and the protein level were observed. Results The infarction volume coincided with the dominative scope of the middle cerebral artery of the electric coagulation. There were significant differences in the cerebral water content as the various treatment groups compared with that of the model group （allP〈0.05）. The VEGF positive cell number and the protein level around the infarction area in the acupoint sticking group were increased as compared with those in the model group （P〈0.01）, with no significant difference as compared with the Nimodipine group and the acupuncture group （allP〉0.05）. Conclusion Acupoint sticking of ＂Huayutie＂ alleviates the cerebral damage after ischemia possibly through enhancing the expression and protein level of VEGF.