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视黄酸X受体α促进猪前体脂肪细胞分化 被引量：2

RXRα Promotes Differentiation of Porcine Preadipocytes
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摘要 采用细胞转染、油红O染色、油红O染色提取法、GPDH活性测定、semi-qRT-PCR等方法研究了视黄酸X受体α(retinoic acid X receptorα,RXRα)在猪原代前体脂肪细胞分化中的作用及其机理.结果表明,转染pRXRα-EGFP促进了猪前体脂肪细胞RXRα的表达,脂肪细胞分化能力随之增强,脂肪细胞GPDH活性、分化转录因子PPARγ和C/EBPα mRNA表达水平均显著升高(P<0.05).结果提示,RXRα可能通过调控过氧化物酶体增殖物激活受体γ(peroxisome proliferator-activated receptor-γ,PPARγ)和CAAT/增强子结合蛋白家族(CCAAT/enhancer binding proteins,C/EBP)C/EBPα基因表达变化促进猪前体脂肪细胞分化. The effects of retinoic acid X receptor α （RXRα） overexpression were studied during the differentiation of porcine adipocytes by various assays, such as plasmid transfection, oil red O extraction, GPDH activity measurement and semi-qRT-PCR. The results showed that with the increased level of pRXRα- EGFP following transfection to primary cultured porcine preadipocytes, preadipocytes differentiation with morphological changes of adipocytes was observed. Meanwhile, GPDH activity and the expression level of differentiation transcription factors PPARγ （peroxisome proliferator-activated receptor-γ ） and C/EBPα （CCAAT/enhancer binding proteins） were significantly increased （P 〈 0.05）. The data implies that RXRα promotes differentiation of porcine adipocytes by enhancing the PPARγ and C/EBPα mediated pathway.
作者 林亚秋 庄和林 杨公社
机构地区 西北农林科技大学动物脂肪沉积与肌肉发育实验室 西南民族大学生命科学与技术学院
出处 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2009年第3期264-269,共6页 Chinese Journal of Biochemistry and Molecular Biology
基金 国家自然科学基金资助资项目(No.30471239)~~
关键词 脂肪细胞 分化 视黄酸X受体α 猪 adipocyte differentiation retinoic acid X receptor α （RXRα） porcine
分类号 Q254 [生物学—细胞生物学]
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参考文献22

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共引文献29

同被引文献35

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中国生物化学与分子生物学报
2009年 第3期

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