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牛IRAK2基因有两种选择性剪接表达产物 被引量：1

Bovine IRAK2 Gene Encodes Two Alternatively Spliced Isoforms
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摘要 Toll样受体(TLRs)可识别病原体相关分子模式,在天然免疫和适应性免疫应答中起着重要的作用.IRAK2为TLRs信号转导中的重要分子,对信号转导及调节有着重要的作用.为了深入研究牛TLRs介导的信号转导通路对牛病原体所致疾病抗性中的作用,本研究采用RT-PCR和RACE方法克隆了牛IRAK2基因,并分析了基因序列,及其编码蛋白质的结构和功能预测.结果表明,牛IRAK2存在2个选择性剪接产物IRAK2a和IRAK2b,其cDNA长度分别为2148bp和2001bp(GenBank登录号为EU528620和EU528621),分别编码622个和384个氨基酸.与IRAK2a相比,IRAK2b缺少第3外显子147bp片段,使得起始密码子后移,IRAK2a蛋白含有DD结构域和S-TKc结构域,而IRAK2b则缺少DD结构域,只有部分S-TKc结构域.
作者 王兴平 罗仍卓么 许尚忠 高雪 李俊雅 任红艳 陈金宝
机构地区 新乡医学院生命科学技术系 中国农业科学院北京畜牧兽医研究所
出处 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2009年第3期292-296,共5页 Chinese Journal of Biochemistry and Molecular Biology
基金 国家高技术研究发展计划(863计划,No.2006AA10Z197) 国家科技支撑计划(No.2006BAD01A10 2006BAD14B07 2006BAD04A16)资助~~
关键词 牛 IL-1R相关激酶-2 信号转导 TOLL样受体
分类号 Q75 [生物学—分子生物学]
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二级参考文献10

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共引文献13

同被引文献27

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中国生物化学与分子生物学报
2009年 第3期

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