垂体腺瘤中瘦素的表达及临床意义

Expression of leptin in pituitary adenomas and its clinical significance

目的检测瘦素在垂体腺瘤中的表达并探讨其与肿瘤侵袭性和增殖能力之间的关系。方法收集上海第二军医大学附属长征医院神经外科自2007年2月至10月手术切除的61例垂体腺瘤标本及患者临床资料，应用免疫组化方法检测瘦素和Ki-67在垂体腺瘤组织中的表达。结果本组垂体腺瘤中瘦素阳性表达率为34．4％（21／61）；瘦素在正常垂体组织中的阳性表达率高于垂体腺瘤；瘦素染色阳性组的Ki-67指数、肿瘤直径低于瘦素染色阴性组；瘦素染色阳性组非侵袭性垂体腺瘤表达率高于瘦素染色阴性组，差异均有统计学意义（P〈0．05）。结论与正常垂体组织相比，垂体腺瘤中瘦素的表达下降；垂体腺瘤中瘦素的表达与垂体腺瘤的大小、侵袭性、增殖系数有关。而与患者的性别、年龄等无关。

Objective To detect the expression of leptin in pituitary adenomas and investigate the association of leptin expression with the invasiveness and proliferation of pituitary adenomas. Methods Sixty-one surgical specimens of pituitary adenomas and the clinical data of the cases were obtained from the Department of Neurosurgery, Changzheng Hospital between February and October in 2007. Immunohistochemical staining was used to detect the expressions of leptin and Ki-67 in the pituitary adenomas to investigate the role of leptin in the occurrence and development of the tumor. Results The overall leptin positivity rate in these pituitary adenomas was 34.4%, significantly lower than that in normal pituitary tissues （P〈0.05）. The Ki-67 index and diameter of the leptin-positive pituitary adenomas were significantly lower than those of leptin-negative tumors （P〈0.05）. The leptin expression rate was significantly lower in invasive pituitary adenomas than in non-invasive adenomas （P〈0.05）. Conlusions Compared with normal pituitary tissues, pituitary adenomas have reduced leptin expression, which is significantly correlated to Ki-67 index, tumor invasiveness and diameter but not to the patients＇ age or gender.