大鼠动情周期子宫及卵巢内SLC26A3的表达

Expression of SLC26A3 in Uterus and Ovary of Rats in Estrous Cycle

目的探讨溶质相关载体26A3(SLC26A3)在大鼠动情周期子宫及卵巢内的表达。方法根据阴道涂片检测结果,将12只雌性Wister大鼠分别在动情前期(P)、动情期(E)、动情后期(M)和动情间期(D)断头处死,取出卵巢及子宫,提取总RNA及总蛋白,用RT-PCR及Western blot方法检测卵巢及子宫内SLC26A3在动情周期中各时期的表达。结果子宫内SLC26A3在动情期mRNA的转录水平和蛋白的表达水平相对较高,卵巢内SLC26A3在动情前期和动情期mRNA的转录水平和蛋白的表达水平相对较高。结论动情周期中,SLC26A3在大鼠卵巢及子宫内呈规律性表达,其变化为受孕做好了充分准备。

Objective To explore the expression of solute linked carrier （SLC） 26A3 in uterus and ovary of rats in estrous cycle. Methods According to the examination result of vagina smears, 12 female rats were killed in proestrus （P）, estrus （E）, metaestms（M） and diestrus （D） respectively, 3 for each, and their uterus and ovary were taken out, from which the total RNA and total protein were extracted for determination of transcription of SLC26A3 mRNA by RT-PCR, and expression of SLC26A3 protein by Western blot. Results The SLC26A3 mRNA transcription and protein expression levels were relatively high in uterus of rats at E phase, and in ovary of rats at P and E phases. Conclusion SLC26A3 was expressed regularly in uterus and ovary of rats in estrous cycle, and the change of expression level provided a condition for impregnation.