摘要
目的研究花生四烯酸(AA)对兔心室肌细胞电压门控钠通道(VGSC)的影响。方法酶解法分离兔心室肌细胞,采用标准全细胞膜片钳技术记录电压门控钠通道电流(INa)。结果AA可浓度依赖性抑制INa,使INa的I-U曲线上移,但其激活电位、电位峰值和反转电位保持不变;AA使INa稳态失活曲线左移,恢复曲线右移;但对INa的抑制作用不具有明显的频率依赖性。结论AA对INa具有浓度依赖性抑制作用,主要是通过抑制失活和失活后恢复过程而发挥作用。因此AA对INa的抑制作用可能是AA抗心律失常,保护心肌的作用机制之一。
Aim To study the effect of arachidonic acid on the vohage-gated sodium channel of rabbit cardiac ventricular myocytes. Methods Single ventricular myocytes of rabbits were obtained by enzymatic dissociation method. And the whole-cell patch-clamp recording technique was used to record the change of voltagegated sodium channel current (lNa). Results Arachidonic acid decreased INa in a dose-dependent manner. AA shifted the INa I-U curves but didn't change their active, peak and reverse potentials; The inactivation curve was moved to more negative potential and the recovery curve was delayed. But AA can not affect INa in a frequency-dependent manner and the activation curve had no significant change. Conclusion AA can block INa in a concentration-dependent manner, and it mainly affects the inactivated state and recovery curve of INa, which may be one of the mechanisms of its antiarrhythmic effect and myocardial preservation.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2009年第3期331-335,共5页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No30570727)
关键词
心室肌细胞
花生四烯酸
膜片钳技术
电压门控钠通道
ventricular myocytes
arachidonic acid
patch-clamp techniques
voltage-gated sodium channel