蜕膜基质细胞条件培养液对滋养细胞浸润功能的影响

Effect of conditioned media from decidual stromal cell cluture on trophoblast cell invasion

目的:探讨蜕膜基质细胞条件培养液(DSCM)对滋养细胞浸润力的影响。方法:体外培养人早孕正常蜕膜基质细胞,收集DSCM处理早孕滋养细胞系(B6Tert)。应用浸润实验分析B6Tert细胞浸润力的改变,采用RT-PCR与明胶酶谱技术检测B6Tert细胞中基质金属蛋白酶(MMPs)的表达变化。结果:DSCM浓度较低时(占细胞培养液5%),可促进B6Tert细胞的浸润力与该细胞MMP-2mRNA及pro-MMP-2的表达;反之,高浓度的DSCM(20%)则抑制滋养细胞的浸润力与MMP-2的表达,差异有统计学意义(P<0.05)。DSCM不影响B6Tert细胞中MMP-9的表达。结论:早孕DSCM可能通过调节滋养细胞中MMP-2的表达影响滋养细胞的浸润能力。

Objective：To explore the effect of decidual stromal cell conditioned media （DSCM） on trophoblast cell invasion. Methods：In vitro culture of decidual stromal cells obtained from healthy women of early pregnancy was performed to prepare DSCM. Trophoblastic cells （B6Tert cell line） were treated with DSCM. Analysis of the invasive ability and MMPs expression in B6Tert cells were performed by transwell invasion assy, RT-PCR and gelatin zymography, respectively. Results： The invasive ability and the MMP-2 expression of B6Tert cells were significantly increased in these cells cultured in medium containing 5% DSCM. However, the invasive ability and the MMP-2 expression of B6Tert cells were significantly reduced in the cells cultured in medium containing 20aA; DSCM（P 〈0.05 ）. No effects of DSCM on MMP-9 expression were detected. Conclusion：The decidual microenvironment may exert the key control for trophoblast cell invasion mainly through influencing MMP-2 expression.