DNA-PK亚单位Ku70、Ku80在不同分期乳腺癌中的表达情况

Expression of DNA- PK Subunit Ku70 and Ku80 in Breast Cancer at Different Stage

目的探讨不同期别乳腺癌组织中DNA依赖蛋白激酶(DNA dependent protein kinase,DNA-PK)的两个调节亚单位Ku70、Ku80的表达变化情况.方法采用免疫组化SP法检测Ⅰ～Ⅳ期乳腺癌组织各30例中的Ku70、Ku80的表达情况.结果Ku70、Ku80在Ⅰ期乳腺癌组织中呈高表达,在Ⅱ期中的表达有降低趋势(与Ⅰ期比较P<0.01,与Ⅲ期比较P>0.05,与Ⅳ期比较P<0.01),在Ⅲ、Ⅳ期乳腺癌组织中的表达明显低于Ⅰ期乳腺癌(P<0.01;P<0.01).结论Ku70、Ku80在不同阶段乳腺癌中的表达情况是随着乳腺癌的分期变化而变化,从早期、中期到晚期呈逐渐降低趋势,可能与乳腺癌的分期及预后有一定相关性.

Objective To investigate the interrelation between the expression of Ku70 and Ku80 in breast cancer tissue and staging. Methods The expression of Ku70 and Ku80 was examined in 30 cases of stage Ⅰbreast cancer tissues, 30 cases of stage Ⅱ breast cancer tissues, 30 cases of stage Ⅲ breast cancer tissues and 30 cases of stage Ⅳ breast cancer tissues by using immunohistochemical SP method. Results The expression levels of Ku70 and Ku80 in stage Ⅰ breast cancer tissues were high. Compared with stage Ⅰ breast cancer, the expression of Ku70 and Ku80 in stage Ⅱbreast cancer was lower （P 〈 0.01 ）. Simultaneously, the expression of Ku70 and Ku80 between stage Ⅱ and stageIV had obvious difference （P 〈 0.01 ）. The expression levels of Ku70 and Ku80 in stage Ⅲ and stage Ⅳ breast cancer tissues were obviously lower than those in stageⅠ breast cancer tissues （P〈 0.01 and P〈0.01）. Conclusions From early breast cancer to advanced breast cancer, there is a tendency that the expression of Ku70 and Ku80 in breast cancer gradually decrease. The low-expression of Ku70 and Ku80 may play a role in the developing of breast cancer and the metastases of breast cancer. Ku70 and Ku80 may be associated with breast cancer staging system and prognosis.