人轮状病毒感染昆明小鼠乳鼠模型的建立

Model Establishment of Neonatal Kunming Mice Infected with Human Rotavirus

目的建立人轮状病毒G3型709株感染4 d龄昆明小鼠乳鼠模型。方法通过灌胃病毒的方式造模,观察乳鼠被病毒攻击后不同时间其临床表现、小肠组织病理改变、小肠组织上皮细胞超微结构改变。酶联免疫吸附法检测轮状病毒抗原在乳鼠粪便中的表达,免疫荧光法检测轮状病毒在乳鼠小肠组织中的表达。结果4 d龄昆明小鼠乳鼠被轮状病毒攻击24 h后出现腹泻表现和小肠组织病理改变,72 h最严重,之后腹泻率下降,病理改变减轻,第7天腹泻停止,病理改变消失。乳鼠小肠上皮细胞出现糖、脂肪代谢紊乱,其粪便和小肠组织中都可以检测出轮状病毒抗原表达。结论4 d龄昆明小鼠乳鼠被人轮状病毒A组G3型709株经口攻击后病毒能够在其体内复制,出现腹泻表现。该病毒感染腹泻过程具有自愈特点。

Objective To establish the neonatal Kunming mice model infected with a human rotavirus. Method Four days old neonatal Kunming mice were inoculated through gavaging. We valued the neonatal mice＇ s clinical manifestation, small intestine pathological changes. We detected the rotavirus antigen in mice stools and small intestines by ELISA and IF respectively. Result Four days old neonatal mice developed diarrhea and intestinal pathological changes 24 hours pest inoculated, the most serious diarrhea rate and pathological changes appeared 72 h, then dropped. The neonatal mice stopped diarrhea at 7d. Infected neonatal mice small intestine epithelial cells showed sugar and lipid metabolism disorder while rotavirus antigen expressed in their small intestines and stools. Conclusion Four days old neonatal Kunming mice infected with group A human rotavirus G3-709 developed diarrhea performance and the virus infected process had a self-healing feature.