亚洲Ⅰ型口蹄疫病毒双拷贝VP1基因的串联表达及其免疫原性

Tandem Expression and Immunogenicity of Two Copies VP1 Gene of FMDV Serotype Asia 1

口蹄疫病毒(FMDV)VP1基因含有T细胞和B细胞表位,是口蹄疫病毒的主要免疫原性基因。作者将亚洲Ⅰ型FMDV VP1基因首尾串联构建双拷贝的VP1基因(2VP1),实现双拷贝VP1基因的原核表达,表达的重组蛋白(GST2VP1)经Sephadex-G200分子筛层析纯化后,Western blot证实其有反应原性,动物试验表明重组蛋白在加强免疫后能够产生和灭活疫苗相当的ELISA抗体和中和抗体;本试验结果为亚洲Ⅰ型FMDV免疫原性研究及GST2VP1蛋白的进一步应用奠定了基础。

VP1 gene of foot-and-mouth disease virus （FMDV） is the major immunogenic gene as it contains T cell and B cell epitopes. In this study, we constructed a recombinant expressing plasmid（KG2VP1） including two VP1 genes（2VP1） of Asia 1 FMDV, and the 2VP1 gene was expressed in E. coli BL21. The fusional protein （GST2VP1） which was purified by Sephadex-G200 sieve chromatography demonstrated the reactionogenicity by Western blot. And the mice test indicated that the GST2VP1 can induce similar levels of ELISA and neutralization antibody like inactivated vaccine. We provide foundation for researching the immunogenicity of FMDV serotype Asia 1 and further application of GST2VP1 in future.