叶黄素对化学诱导急性肝损伤小鼠的预防研究

Study on protective effect of lutein on acute chemical liver injury in mice

目的研究叶黄素对四氯化碳(CCl4)所致急性化学性肝损伤小鼠的保护作用。方法雄性昆明种小鼠60只,随机分为正常对照组、急性化学性肝损伤模型组(模型组)、联苯双酯阳性对照组(阳性对照组)(联苯双酯15mg/kg)以及叶黄素低、中、高剂量组(10、15、20mg/kg)共6组,每组10只。测定并比较各组小鼠肝脏系数,血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性与丙二醛(MDA)含量;测定肝组织中SOD、GSH-Px活性、MDA含量以及组织病理系数。结果叶黄素各剂量组均能升高急性化学性肝损伤小鼠血清与肝组织SOD、GSH-Px活性(P<0.01),降低血清ALT、AST活性(P<0.01)和血清与肝组织MDA含量(P<0.01),并能不同程度地改善肝脏病理组织损伤。结论叶黄素对CCl4所致急性化学性肝损伤具有预防性保护作用。

Objective To study the protective effect of lutein on the acute chemical liver injury induced by CCl4 in mice and investigate its possible mechanisms. Methods 60 male Kunming mice were divided into control, acute chemical liver injury model, DDB （ 15 mg/ kg） and lutein low （ 10 mg/kg） , middle （ 15 mg/kg） , high dose （20 mg/kg） groups. The liver index and the levels of ALT and AST were tested. The expressions of serum and liver tissue superoxide dismutase （SOD） , maloudialdehyde （MDA） , glutathione perioxidase （ GSH- Px） and liver tissue pathology index were measured. Results Different dose lutein significantly increased the levels of serum and liver tissue SOD and GSH-Px （P 〈0. 01 ）, decreased the levels of ALT, AST （P 〈0. 01 ） and MDA both in serum and liver tissue （P 〈0.01 ）. Lutein improved the liver injury in different degree. Conclusions Lutein has protective effect on the acute chemical liver injury induced by CCl4.