Smad7对脑胶质母细胞瘤U251细胞生物学行为的影响
摘要
目的观测Smad7对脑胶质母细胞瘤U251细胞增殖及浸润的影响。方法应用CCK-8和RT-PCR分别检测Smad7稳定转染U251细胞与空载体转染U251细胞及U251细胞在增殖和N-cadherin、E-cadherin、vimentin、twist基因表达的差异。结果Smad7转染后,上皮细胞间质化相关基因表达未见改变(P>0.05),但相对于空载体转染U251细胞及U251细胞其增殖速度减慢。结论Smad7稳定转染后未见胶质母细胞瘤上皮细胞间质化改变,但Smad7高表达可抑制胶质母细胞瘤细胞增殖。
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2009年第6期693-695,共3页
Chinese Journal of Gerontology
参考文献9
-
1Naumann U,Maass P,Gleske AK,et al. Glioma gene therapy with soluble transforming growth factor-beta receptors Ⅱ and Ⅲ〔J〕.Int J Oncol,2008;33:759-65.
-
2Vega EA,Graner MW,Sampson JH.Combating immunosuppression in glioma〔J〕.Future Oncol,2008;4:433-42.
-
3Rainov NG,Soling A.Clinical studies with targeted toxins in malignant glioma〔J〕.Rev Recent Clin Trials,2006;1:119-31.
-
4Filyak Y,Filyak O,Souchelnytskyi S,et al. Doxorubicin inhibits TGF-beta signaling in human lung carcinoma A549 cells〔J〕.Eur J Pharmacol,2008;590:67-73.
-
5Broderick P,Carvajal-Carmona L,Pittman AM,et al.A genome-wide association study shows that common alleles of SMAD7 influence colorectal cancer risk〔J〕.Nat Genet,2007;39:1315-7.
-
6Bornstein S,Hoot K,Han GW,et al.Distinct roles of individual Smads in skin carcinogenesis〔J〕.Mol Carcinog,2007;46:660-4.
-
7Hong S,Lee C,Kim SJ.Smad7 sensitizes tumor necrosis factor induced apoptosis through the inhibition of antiapoptotic gene expression by suppressing activation of the nuclear factor-kappaB pathway〔J〕.Cancer Res,2007;67:9577-83.
-
8Javelaud D,Mohammad KS,McKenna CR,et al. Stable overexpression of Smad7 in human melanoma cells impairs bone metastasis〔J〕.Cancer Res,2007;67:2317-24.
-
9Kimura ET,Matsuo SE,Ricarte-Filho JC. TGFbeta,activin and SMAD signalling in thyroid cancer〔J〕.Arq Bras Endocrinol Metabol,2007;51:683-9.
-
1张晓明,杨立春,胡韶山.光动力疗法对C6胶质瘤细胞转化生长因子-β的影响[J].神经疾病与精神卫生,2013(4):339-341.
-
2王艳芬,王东林.CD105与肿瘤血管形成[J].南通大学学报(医学版),2006,26(1):75-76. 被引量:1
-
3陈国平,夏立平.TGF-β/Smads信号转导途径与肿瘤的关系[J].海南医学院学报,2009,15(1):91-93. 被引量:4
-
4姜永冬,陈艳波,何川,潘薇,庞达,张国强.hCLP46基因在不同侵袭转移潜能乳腺癌细胞表达中的差异及其意义[J].实用肿瘤学杂志,2012,26(4):336-339. 被引量:1
-
5游颜杰,毕磊,庄轶轩,邱希辉,张灏.小干扰RNA下调乳腺癌扩增基因3表达对乳腺癌MDA-MB-231细胞转化生长因子-β通路的影响[J].转化医学杂志,2012,1(3):144-146. 被引量:1
-
6孙江龄,何常,刘俊玲,程明亮.酒精性肝纤维化及肝癌大鼠Klf6及基因的表达[J].贵阳医学院学报,2013,38(6):588-592. 被引量:2
-
7王晓峰,武文婷,常延超,武涛涛,王飞,孙然,师晨霞.骨关节炎软骨细胞电压依赖性钙通道α1亚单位表达的改变[J].中华实验外科杂志,2016,33(7):1727-1730. 被引量:1