老年上皮性卵巢癌组织中磷酸化AKT和P-gp表达与化疗反应及预后的关系

Correlation between the expressions of p-AKT,P-gp and chemotherapy effects and prognosis in epithelial ovarian cancer tissue

目的探讨老年上皮性卵巢癌组织中磷酸化-AKT(p-AKT)和P-糖蛋白(P-gp)表达与卵巢癌化疗反应及预后的关系。方法采用免疫组化SP法检测63例上皮性卵巢癌组织和20例正常卵巢组织中p-AKT和P-gp表达,分析其与化疗反应的关系,同时对预后进行多因素的Cox生存分析。结果p-AKT和P-gp在上皮性卵巢癌组织中阳性表达率分别为52.4%和31.7%,显著高于正常卵巢组织(P<0.01),且p-AKT和P-gp表达存在相关性。p-AKT和P-gp表达阳性组的化疗反应率显著高于p-AKT和P-gp表达阴性组(P<0.001)。多因素生存分析显示,年龄≥60岁、手术病理分期、术后残留灶直径≥2cm是影响患者预后的独立危险因素。结论p-AKT和P-gp阳性表达与上皮性卵巢癌组织化疗耐药有关,p-AKT过表达对P-gp阳性表达可能具有促进作用,在卵巢癌化疗耐药中起重要作用,对合理制定化疗方案、预测化疗反应性及估计预后具有积极的指导意义。

Objective To investigate the relationship between the expressions of phosphorylation AKT（ p-AKT）, P-gp and chemotherapy effects and prognosis in eldly epithelial ovarian cancer tissue. Methods The expressions of p-AKT and P-gp in 63 ovarian cancer tissues and 20 normal ovarian tissues were tested by immunohistochemistry. Results The positive rates of p-AKT （ 52.4% ） and P-gp （31.7%） in ovarian cancer tissue were higher than those in normal ovarian tissue（ P 〈 0. 01 ）, and there was positive correlation between p- AKT and P-gp. The chemotherapy reaction rates of p-AKT and P-gp positive groups were higher than those of p-AKT and P-gp negative groups （P 〈 0. 001 ） . Aging（ ≥60 years old） , pathologic stage and diameter of surgery focal residual ≥2 cm were independent risk factors influencing prognosis. Conclusions The expressions of p-AKT and P-gp are related with drug fast of epithelial ovarian cancer chemotherapy. The overexpression of p-AKT may promote the expression of P-gp.