宫颈癌细胞的HCS基因辐射效应的研究

Effects of Irradiation on HCS Gene Expression in Hela Cells

目的:应用实时PCR技术分析60Coγ射线照射对宫颈癌(Hela)细胞HCS基因表达的影响。方法:对离体培养的宫颈癌细胞,以0.5Gy、2Gy、4Gy、6Gy、10Gy、15Gy等不同剂量60Coγ射线照射后4小时进行HCS基因表达量测试,分析辐射剂量效应;选择2Gy、10Gy辐射剂量辐射后2小时、4小时、8小时、12小时、24小时等不同时间点分别进行HCS基因表达量测试,分析辐射时间效应。结果:对于Hela细胞HCS基因相对表达量随着辐射剂量的增大,在0.5Gy和6Gy时出现两个高峰,在10Gy、15Gy时出现了再次上升的态势;Hela细胞对2Gy与10Gy的辐射显现相近的辐射后时间效应,在辐射后4～8小时出现基因表达的最大值。结论:HCS基因有可能成为人类宫颈癌辐射损伤生物分子的一个标记物,对宫颈癌放疗具有潜在的应用价值。

Objective： Using real-time PCR, Hela cells exposed to γ-rays of cobalt 60 were investigated for holocarboxylase synthetase（HCS） gene expression.Methods：Cultured Hela cells were exposed to y-rays at the doses of 0.5,2,4,6,10,15 Gy respectively for four hours. After irradiation,the transcription levels of HCS gene were determined and analyzed for the dosage effect of radiation. We also measured the transcription levels of HCS gene 2,4, 8,12,24 hours after the irradiation at the doses of 2 Gy and 10 Gy, and analyzed the time course effect post-radiation. Results： As the radiation dosage increases for Heia cells, HCS gene relative expression reached two high peaks at the doses of 0.5 Gy and 6 Gy, and it also demonstrate trends to increase at the doses of 10 Gy and 15 Gy. For time course effect post-radiation, there was no significant difference for Heia cells＇ response between the 2 Gy and the 10 Gy treatment groups. The maximum amount of gene expression was observed 4 - 8 h after irradiation in both groups. Conclusions： HCS gene expression could be a candidate biomarker to detect radiation injury, and it also has the potential application value in radiation-therapy for cervical carcinoma.