子宫腺肌病患者内膜组织中PTTG的表达及与MMP-2和TIMP-1的关系

PTTG Expression and Its Relationship with MMP-2,TIMP-1 in Adenomyosis

目的:检测子宫腺肌病患者内膜组织中垂体肿瘤转化基因(PTTG)的表达,并分析其与基质金属蛋白-2(MMP-2)、基质金属蛋白酶组织抑制因子-1(TIMP-1)的相关性。方法:采用免疫组化SP方法检测35例子宫腺肌病组、在位内膜组和24例非子宫腺肌病患者正常内膜组中PTTG、MMP-2及TIMP-1蛋白的表达水平。结果:①PTTG在子宫腺肌病组、在位内膜组和正常内膜组间的表达差异有高度统计学意义(P<0.001),其中子宫腺肌病组和在位内膜组表达强度显著高于正常内膜组(P<0.001),子宫腺肌病组与在位内膜组间差异无统计学意义(P>0.05);②MMP-2在子宫腺肌病组和在位内膜组中的表达强度高于正常内膜组(P<0.05);③TIMP-1在子宫腺肌病组中的表达比正常内膜组低,两组相比差异有高度统计学意义(P<0.001);④PTTG与MMP-2的表达呈正相关,与TIMP-1的表达无相关性。结论:子宫腺肌病内膜中PTTG高表达,导致MMP-2表达上调,并可能影响TIMP-1的表达,从而使子宫内膜的增殖活性增强、侵袭力增高,致使子宫内膜向下浸润性生长,提示PTTG参与了子宫腺肌病的发生。

Objective： To investigate expression of pituitary tumor transforming gene （PTTG）, and its correlation with expression of matrix metallopreteinase-2 （MMP-2） and tissue inhibitor of metalloproteinases-1 （ TIMP-1 ） in adenomyotic tissue. Methods： The expression of PTTG, MMP-2, and TIMP-1 were detected by immunohistochemistry using SP in 35 cases of the adenomyotic tissue and eutopic endomatrium and 24 cases of normal endometrial tissue. Results： Expression of PTTG had statistic difference in the adenomyotic tissue, eutopic enclometrium, and normal endometdum （ P 〈 0.001 ）. PTTG expression in the adenomyotic tissue and eutopic endometrium was higher than normal endomatrium （ P 〈 0.001 ）, but there was no difference between them （ P 〉 0.05）. The expression of MMP- 2 in the adenomyotic tissue and eutopic endometrium was higher than that of in the normal endomatrlal tissue （ P 〈 0.05）. TIMP-1 expression in the adenomyotic tissue was statistically lower than in the normal endomatrium （ P 〈 0.001）. PTTG expression had correlation with MMP-2, but not TIMP-1. Conclusions： Higher expressions of PTTG in adenomyotic endometrium will up-regulate MMP-2 and influence TIMP-1 expression. Therefore, endometrium enhances proliferation and invasion, finally result in the infiltrative growth of endometrium. PTTG may play an important role in the development of adenomyosis.