脊髓髓外冲击负荷后脊髓血流与诱发电位变化的相关研究

Relevant research of spinal cord blood flow and the evoked potential by the extramarrow impacting

[目的]研究脊髓髓外冲击负荷对脊髓血流及诱发电位的影响,探讨脊髓损伤后脊髓血流与诱发电位变化的规律,以及它们之间的相关性。[方法]选用雄性SD大鼠120只,随机分为4组,每组30只,A组为假手术组,B、C、D为实验组,在大鼠T10椎板处分别给予10、15、20 N的椎板外冲击负荷。术后0 min,1、6 h,1、6、12、24d时间点测量冲击负荷位置的脊髓白质、灰质血流以及SEP、MEP测量,进行相关性分析。[结果]脊髓内灰质白质的血流表现出随冲击负荷的增加而减小的趋势。A组脊髓血流及SEP、MEP无明显变化,B组脊髓血流及SEP、MEP出现一过性降低然后恢复正常,两组之间无明显差异。C、D组脊髓血流及SEP、MEP均表现为持续下降后再缓慢的回升的规律性变化,其中C组在24 d恢复正常,D组在24 d时仍然低于正常,经统计学分析脊髓血流与诱发电位具有明显的相关性。[结论]椎板外冲击负荷通过改变脊髓血流来影响脊髓的功能,并且脊髓血流和诱发电位有明显的相关性。

[ Objective ] To research the influence of spinal cord blood flow （SCBF） and the evoked potential （EP） by the extramarrow impacting and to explore the correlation and regularity between the spinal cord blood flow and evoked potential after spinal cord injury. [ Method] Normal 120 SD rats were randomized into 4 groups,with 30 in each. Group A was the control group. In groups B, C, D,impacts of 10,15,20N were given to lamina respectively. The blood flow of impacting position and evoked potential were＇observed at different time points including 0 min, 1 h,6 h, 1 d,6 d, 12 d,24 d. [ Result] The blood flow of spinal cord represents a dimning trend with the impacting load raising. The blood flow of group A, had no marked change,the SEP and MEP also. The blood flow of group B represented a temporaly lowing , then recovered normal, the SEP and MEP also. The blood flow of group C and D represented a continuous lowing , then slowly raising. The group C recovered normal at 24d, the group D still lower than normal. [ Conclusion ] The impact load to lamina can influence the spinal cord function through altering the spinal cord blood flow. There is evident correlation between the SCBF and EP.