炎症因子在大鼠慢性牙周炎合并动脉粥样硬化模型中的表达

Expression of inflammatory cytokines in the model of chronic periodontitis associated with atherosclerosis of rats

目的:检测和分析大鼠慢性牙周炎(chronic periodontitis,CP)合并动脉粥样硬化(atheroscle-rosis,As)模型中牙周组织白细胞介素-6(Interleukin-6,IL-6)和血清C反应蛋白(C-reactive protein,CRP)的表达。方法:60只成年Wistar雄鼠随机分为4组,每组15只,A为正常对照组,B为CP组,C为As组,D为CP+As组;各组接受相应的建模处理。观察牙周组织和动脉血管病理改变,免疫组织化学方法半定量分析牙周组织IL-6表达,酶联免疫吸附法(ELISA)检测血清CRP水平。结果:牙周病理学观察,B、D组实验牙牙周炎症表现明显,附着丧失(AL)水平较A、C组有明显增加(P<0.05)。动脉病理学观察,C、D组主动脉和胸、腹主动脉形成粥样硬化病变。B、D组牙周组织IL-6表达和血清CRP水平均高于其他组(P<0.05)。相关性分析显示,B、D组血清CRP与牙周组织IL-6均呈正相关。结论:CP与As可能相互影响、促进,两疾病的发生发展与炎症因子表达升高有关。

AIM： To detect and analyse interleukin -6 in periodontal tissue and serum CRP levels in the model of chronic periodontitis associated with atherosclerosis of rats. METHODS： Sixty adult male Wistar rats were randomly divided into four groups ： group A （ control group）, group B （ periodontitis group）, group C （ atherosclerosis group） and group D （periodontitis associated with atherosclerosis group）. Every group accepted the corresponding treatment. Pathological changes in periodontal tissue of experimental teeth and arterial vessels were observed. Semi - quantitate analyss of IL -6 in periodontal tissue was done by immunohistochemistry method. CRP level in serum was assayed by ELISA. RESULTS ： Histopathology observation of periodontal tissue was as follows. Obvious inflammation of periodontal tissue was observed in group B and D. Attachment loss level in group B and D was higher than that in group A and C（P 〈0.05）. Histopathology observation of arteries revealed that tunica media of artery thickened in group B and that thoracic aorta and abdominal aorta atherosclerotic lesions wene formed in group C and D. IL -6 in periodontal tissue and serum levels of CRP in group B and D were higher than in other groups （P 〈 0.05）. Dependablity analyse showed that CRP and IL -6 in both group B and D were positive correlation. CONCLUSION： Atherosclerosis and periodontitis could influence each other, the development of two diseases may concerne with elevated levels of inflammatory factors.