褪色光度法测定透明质酸钠研究

Fading Spectrophotometric Determination of Sodium Hyaluronate

在近中性介质中，基于甲基紫与透明质酸钠发生缔合作用使甲基紫溶液褪色，建立了甲基紫分光光度测定透明质酸钠的新方法，确定了测定的最佳条件。实验结果表明，透明质酸钠质量浓度在25-175μg／10mL范围内服从比尔定律，其回归方程为A=0．0036ρ（μg／10mL）＋0．0229，相关系数γ=0．9950。所拟方法用于滴眼液中透明质酸钠的测定，结果满意，加标回收率在98．82％～102．5％。

In the medium close to neutrality, the content of sodium hyaluronate in preparations was determined by the methyl violet spectrophotometric method in which methyl violet reacted rapidly with sodium hyaluronate to form complexes, and changes of absorption spectra were caused. The optimum conditions of the sodium hyaluronate were investigated. Beer＇s law was obeyed in the concentration range of 25- 175 μg sodium hyaluronate per 10 mL with the regressive equation of A=0. 003 6ρ（μg/10 mL）＋0. 022 9 as well as the coefficient γ of 0. 995 0. The proposed method was applied to determine sodium hyaluronate in preparations with the recovery rate of 98.82% to 102.5%.