期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

一株低温碱性脂肪酶产生菌TS182的分离与鉴定研究 被引量：1

Screening and Identification of a Strain TS182 Producing a Cold-adaptive Alkaline Lipase
下载PDF
导出
摘要 通过Tween-80平板初筛和摇瓶发酵复筛,从连云港海域海水及海泥样品中分离的菌株中筛选获得一株低温碱性脂肪酶高产菌株TS182。通过形态学、生理生化以及分子生物学鉴定菌株TS182为荧光假单孢菌(Pseudomonas fluorescens)。菌株TS182所产脂肪酶最适温度和最适pH分别为15℃和9.0。 Strain TS182, which produces alkaline lipase, was isolated from strains in the marine water and marine mud collected from Lianyungang. It was identified as Pseudomonas fluorescens by morphological, physiological, biochemical tests and 16S rDNA gene sequence analysis. The optimal temperature and pH of the lipase of strain TS182 were 25 ℃ and 9.0 respectively.
作者 刘姝 房耀维 王淑军 吕明生 陈丽 焦豫良
机构地区 淮海工学院海洋学院
出处 《淮海工学院学报（自然科学版）》 CAS 2009年第1期83-86,共4页 Journal of Huaihai Institute of Technology:Natural Sciences Edition
基金 江苏省高校自然科学基础研究项目(08KJB550001) 连云港市科技公关项目(CG0714) 淮海工学院博士启动基金资助项目(KQ07103)
关键词 低温碱性脂肪酶 荧光假单孢菌 筛选 鉴定 cold-adaptive alkaline lipase Pseudornonas fluorescens screening identification
分类号 Q936 [生物学—微生物学]
  • 相关文献

参考文献10

  • 1QUYEN D T,SCHMIDT-DANNERT C,SCHMID R D.High-level expression of a lipase from Bacillus thermocatenulatus BTL2 in Pichiapastoris and some properties of the recombinant lipase[J]. Protein Expression and Purification,2003,28(1) : 102-110.
  • 2TRIPATHI M K,ROY U,JINWAL U K,et al. Cloning, sequencing and structural features of a novel Streptococcus lipase[J]. Enzyme and Microbial Technology, 2004,34 (5) : 437-445.
  • 3ABBAS H, HIOL A, DEYRIS V, et al. Isolation and characterization of an extracellular lipase from Mucor sp. strain isolated from palm fruit[J]. Enzyme and Microbial Technology, 2002,31 (7) : 968-975.
  • 4李艳华,张利平.海洋微生物资源的开发与利用[J].微生物学通报,2003,30(3):113-114. 被引量：29
  • 5董宏伟,孙谧,王跃军,于建生.海洋微生物低温碱性脂肪酶的纯化与性质研究[J].海洋与湖沼,2004,35(4):376-383. 被引量：16
  • 6HOLT J G,KRIEG N R,SNEATH P,et al. Bergey's Manual of Determinative Bacteriology[M]. 9th ed. Baltimore:The Williams & Wilkins Co. , 1994.
  • 7SAMBROOK J,FRISCH E R,MANIATIS T. Molecular Cloning: a Laboratory Manual[M]. 2nd ed. New York:Cold Spring Harbor Laboratory Press, 1989.
  • 8LEE D W,KOH Y S,KIM K J, et al. Isolation and characterization of a thermophilic lipase from Bacillus thermoleovorans ID-1[J]. Federation of European Microbiological Societies Microbiology Letter, 1999, 179 (2) : 393-400.
  • 9MARSHALL C J. Cold-adapted enzymes[J]. Trends Biotechnol, 1997,15 (9) :359-364.
  • 10邵铁娟,孙谧,郑家声,王跃军,邱秀宝.Bohaisea-9145海洋低温碱性脂肪酶研究[J].微生物学报,2004,44(6):789-793. 被引量：29

二级参考文献37

  • 1QB/T1803-93:0-22.中华人民共和国行业标准工业酶制剂通用试验方法.[S].中华人民共和国轻工业部,1993..
  • 2张树政主编,1984. 酶制剂工业(下). 北京: 科学出版社,655-670
  • 3Abel H,Marie D Jonzo,Danielle Druet et al,1999. Production,purification and characterization of an extracellular lipase from Mucor hiemalis f. hiemalis. Enzyme and Microbial Technology,25: 80-87
  • 4Alquati C,De Gioia L,Santarossa G et al,2002. The cold-active lipase of Pseudomonas fragi. Heterologous expression,biochemical characterization and molecular modeling. Eur J Biochem,269(13): 3321-3328
  • 5Chandrasekaran M,1997. Industrial enzymes from marine microorganisms: The Indian scenario. J Marine Biotechnology,23(5): 0086-0089
  • 6Kamini N R,Fujii T,Kurosu T et al,2000. Production,purification and characterization of an extracellular lipase from the yeast,Cryptococcus sp. S-2. Process Biochemistry,36: 317-324
  • 7Laemmli U K,1970. Cleavage of structural proteins during the assembly of the head. Nature,227: 680-685
  • 8Lee Dong-Woo,Koh You-Seok,Kim Ki-Jun et al,1999. Isolation and characterization of a thermophilic lipase from Bacillus thermoleovorans ID-1. FEMS Microbiology Letters,179: 393-400
  • 9Lin Shuen-Fuh,Chiou Chien-Ming,Yeh Chuan-Mei,1996. Purification and partial characterization of an alkaline lipase from Pseudomonas pseudoalcaligenes F-111. Appl Environmental Microbiol,62(3): 1093-1095
  • 10Lowry O H,Rosebrough N J,Farr A L et al,1951. Protein measurement with the phenol folin reagent. J Biol Chem,193: 265-275

共引文献64

同被引文献14

  • 1孙志浩,柳志强.酶的定向进化及其应用[J].生物加工过程,2005,3(3):7-13. 被引量：7
  • 2CALDWELL R C,JOYEE G F.Randomization of genes by PCR mutagenesis[J].PCR Methods and Applications,1992,2 (1):28-33.
  • 3ZHANG Yinxin,PERRY K,VINCI V A,et al.Genome shuffling leads to rapid phenotypic improvement in bacteria[J].Nature,2002,415:644-646.
  • 4WANG Dai,ZHAO Cheng,CHENG Rong,et al.Estimation of the mutation rate during error-prone polymerase chain reaction[J].Journal of Computational Biology,2000(7):143-158.
  • 5ZHAO Huimin,ARNOLD F H.Optimization of DNA shuffling for high fidelity recombination[J].Nucleic Acids Research.1997,25(6):1307-1308.
  • 6WANG Shujun,LU Zhaoxin,LU Mingsheng,et al.Identification of archaeon producing hyperthermophilic α-amylase and characterization of the α-Amylase[J].Applied Microbiology and Biotechnology,2008,80 (4):605-614.
  • 7KEOHAVONG P,THILLY W G.Fidelity of DNA polymerases in DNA amplification[J].Proceedings of the National Academy of Sciences,1989,86 (23):9253-9257.
  • 8SAIKI R K,GELFAND D H,STOFFEL S,et al.Primer-directed cloning enzymatic amplification of DNA with a thermostable DNA polymerase[J].Science,1988,239:487.
  • 9HIGUCHI R.Simple and rapid preparation of samples for PCR[M]//ERLICH H A.PCR Technology:Principles and Applications for DNA Amplification.New York:Stockton Press,1989:31-38.
  • 10LIN-GOERKE J L,ROBBINS D J,BUREZAK J D.PCR-based random mutagenesis using manganese and reduced dNTP concentration[J].Biotechniques,1997,23(3):409-412.

引证文献1

二级引证文献2

淮海工学院学报（自然科学版）
2009年 第1期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部