集成一次性微流控芯片的便携式荧光检测系统(英文)

Portable Fluorescence Detection System Integrated with Disposable Microfluidic Chip

本文研究了一种新型的生物化学分析系统，该系统包括便携式荧光检测仪和带光纤的微流控芯片．采用基于MEMS技术的微泵将待测物与荧光试剂的混合物导入微流控芯片，采用PMT检测受激发产生的荧光，荧光强度与待测物浓度成一定比例．激发光则通过光纤将光源LED光信号导入微沟道中．随着液体在微沟道中的流动，可连续分析和检测不同的样品．该系统检测1～1000μg／L浓度的荧光素具有0．966的相关系数．基于荧光猝灭原理，该系统还可检测浓度为5ng／μL的硝基化合物．该生化分析系统除具有便携式和一次性微流控芯片优点外，还具有成本低．试剂、样品消耗量少，且分析时间短等优点该系统能实现现场检测，可应用于临床诊断。

A novel biochemical analysis system including a portable fluorescence detection instrument and a microfluidic chip integrated with an optical fiber was developed. When fluid sample containing analyte and fluorescent reagent was transported to the microfluidic chip by a micro-electro-mechanical system （MEMS）-based micropump, the fluorescent light, which was induced by the excitation light from light emitting diode （LED） coupled into the microchannel through an optical fiber, was detected by photomultiplier tube （PMT）. The intensity of fluorescent light is related to the concentration of the analyte. Along with fluid flowing in the microchannel, different samples could be continually detected and analyzed. The system was able to detect fluorescein within the concentration range from 1 μg/L to 1 000 μg/L and the correlation coefficient was 0.966. And then 5 ng/μL nitro compound solution was detected using this system based on the principle of fluorescence quenching. This biochemical analysis system has several advantages of lower cost, lower reagent and sample consumption, and less analytical time besides portability and disposability, which enables the implementation of on-site detection in the fields of point-of-care clinical testing, environmental testing, and biological warfare agent detection.