十和田近等基因系糙米锌含量QTL定位

Identification of QTLs about Zinc Content in Brown Rice for Near-isogenic Lines for Towada

以十和田为轮回亲本,丽粳2号为供体亲本培育出糙米锌含量近等基因系群体BC5F6为材料,从遍布水稻12条染色体上的600对引物中筛选到一个与糙米锌含量有关的SSR标记RM4608。根据其在水稻染色体上的位置,结合PCR扩增结果又发现了与糙米锌含量有关的4个SSR标记(RM19491,RM19489,RM6119和RM19487)。用MAPMAKER3.0软件做出了这5个标记的连锁群,最后采用混合线性模型定位法找到了糙米锌含量的QTL位点。QTL分析结果显示:该位点位于6号染色体上RM4608和RM6119标记之间,贡献率为5%,为新发现的与糙米锌含量有关的微效基因位点,暂命名为qZINC-6。同时与糙米铬和镁含量有关的QTL位点也被发现,其中糙米铬含量QTL位于标记RM19489和RM19491之间,贡献率为9%,是一个主效基因;糙米镁含量QTL位于标记RM4608-RM6119之间,是一个微效基因,贡献率为4%。

With the Near-isogenic lines （BCsF6） populations of zinc content in brown rice （which were bred with Towada as recurrent parent and Lijing2 as donor parent） as experiment material, a SSR marker RM4608, which correlated with zinc content in brown rice, was found from 600 primers distributed in 12 chromosomes office. According to its position in rice chromosome and PCR amplification results, we also found four SSR markers （RM19491, RM19489, RM6119 and RM19487） which correlated with zinc content in brown rice. We use software MAPMAKER3.0 to work out a linkage group of the five markers, with the mixed linear model mapping method, finally we found the locus of QTL related to zinc content in brown rice. QTL analysis showed that this locus was between RM4608 and RM6119 of the sixth chromosome and its percentage of variance explained was 5%. It was a newly-found mini-efficient gene related to zinc content in brown rice and was named as qZINC-6. Meanwhile QTL loci related to chromium and magnesium content in brown rice were also found the QTL related to chromium content in brown rice located between RM19489 and RM19491, as a major-efficent gene, it＇s percentage of variance explained was 9%. The QTL related to magnesium content in brown rice located between RM4608 and RM6119, as a mini-efficient gene, its percentage of variance explained was 4%.