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白菜型油菜PABP5基因启动子的克隆及表达特性分析 被引量：2

Isolation of a Brassica rapa Promoter of Gene PABP5 and its Function Analysis in Transgenic Arabidopsis thaliana
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摘要 本研究中利用基因组步移法从白菜型油菜中克隆到Poly(A)结合蛋白基因PABP5起始密码子上游长588bp的一段序列,经软件分析预测表明,该序列具有典型的启动子结构,并且具有多个与花药特异性表达相关的顺式作用元件,如TATA box,CAAT box,AGAAA motif,AGGTCA motif,AAACCCTAA motif,GTGA motif等。为了研究该片段的表达特性,本文将克隆到的序列置换pPBI121中的CaMV35S启动子,驱动其下游的GUS基因,构建了植物表达载体,转化拟南芥,获得了转基因植株。组织化学染色表明,在PABP5启动子Ppabp5的驱动下,报告基因GUS在拟南芥的根尖、第一片真叶叶原基以及花药中特异表达。本研究为PABP5基因的进一步功能研究奠定了基础。 5＇ flanking sequence ofBrassica rapa Poly（A） binding protein gene PA BP5 was cloned by using PCR-based genomic walking method. Results of sequence analysis showed that the cloned sequence contained several putative cis-elements for anther-specific activity, such as TATA box, CAAT box, AGAAA motif, AGGTCA motif, AAACCCTAA motif, GTGA motif, GCGGGAAA motif and GGGCC motif, as well as several motif for ABA responding. A plant expression vector containing a Ppabp5-GUS fusion construct was constructed and transferred into Arabidopsis thaliana. Histochemical staining suggested that GUS gene express in anther, root tip and apical meristem of the first-true leaf.
作者 石磊 董彩华 白泽涛 郭学兰 柴国华 黄军艳 刘胜毅
机构地区 中国农业科学院油料作物研究所
出处 《分子植物育种》 CAS CSCD 2009年第2期341-346,共6页 Molecular Plant Breeding
基金 国家自然科学基金项目(30671119) 863计划(2006AA10A113)共同资助
关键词 白菜型油菜 PABP5 启动子 拟南芥 花药特异性表达 Brassica rapa, PA BP5, Promoter, A rabidopsis thaliana, Anther-specific expression
分类号 S634 [农业科学—蔬菜学]
  • 相关文献

参考文献16

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二级参考文献14

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分子植物育种
2009年 第2期

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