摘要
目的:检测CXCR4在胃肠道肿瘤中的表达状态,探讨CXCR4在胃肠道肿瘤发病中的作用.方法:采用即时定量PCR(Real-time PCR)检测胃肠道肿瘤标本中CXCR4的表达.采用逆转录PCR(RT-PCR)和Western blot检测CXCR4在胃肠道肿瘤细胞系中的表达.结果:CXCR4mRNA在24例结直肠癌组织标本的表达水平显著高于其配对的癌旁正常组织(P<0.001).CXCR4mRNA在30例胃癌组织标本中的表达水平亦显著高于其配对的癌旁正常组织(P<0.001).CXCR4mRNA和蛋白质在结肠癌细胞系HT-29和SW-480及胃癌细胞系SGC-7901和AGS中均存在明显表达CXCR4表达与胃癌患者分期及淋巴结转移状态具有相关性(P=0.01,0.02).结论:CXCR4在胃肠道肿瘤中存在过度表达,其参与了胃肠道肿瘤的发病过程.
AIM： To determine the expression status of chemokine receptor CXCR4 in gastrointestinal cancer and to explore its role in gastrointestinal carcinogenesis.
METHODS： Real-time PCR was used to detect mRNA expression of CXCR4 in gastrointestinal cancer samples. Reverse-transcription PCR （RT- PCR） and Western blot were used to determine the expression of CXCR4 in gastrointestinal cancer cell lines.
RESULTS： Level of CXCR4 mRNA was sig- nificantly higher in 24 colorectal cancer samples than in matched normal tissues （P 〈 0.001）. Level of CXCR4 mRNA was also significantly higher in 30 gastric cancer samples than in matched normal tissues （P 〈 0.001）. Both CXCR4 mRNA and CXCR4 protein were expressed strongly in colon cell lines HT-29 and SW-480, and gastric cell lines SGC-7901 and AGS. CXCR4 expression was correlated significantly with staging and lymph node metastasis in gastric cancers （P = 0.01 and P -- 0.02, respectively）.
CONCLUSION： CXCR4 is over-expressed in gastrointestinal cancers, and plays a role in gastrointestinal carcinogenesis.
出处
《世界华人消化杂志》
CAS
北大核心
2009年第4期421-424,共4页
World Chinese Journal of Digestology
