槐耳清膏联合化疗栓塞对兔VX2肝癌肝功能、VEGF及MVD的影响

Effect of Huaier extract combined with chemoembolization on the expression of VEGF, MVD and hepatic function of rabbit VX2 hepatocarcinoma

目的:观察槐耳清膏联合经肝动脉化疗栓塞对兔VX2肝癌肝功能、VEGF及MVD的影响.方法:将36只MRI证实已成功接种VX2肝癌的荷瘤兔随机分为3组,开腹经肝动脉穿刺分别给予不同处理:A组为生理盐水对照组,经肝动脉注入0.2mL/kg体质量生理盐水;B组为TACE组,经肝动脉注入超液态碘化油0.2mL/kg+丝裂霉素0.5mg/kg体质量乳剂;C组为TACE+槐耳清膏灌服组,TACE方法同B组,同时术后每天灌服槐耳清膏500mg/kg体质量.介入治疗前1d及治疗后3、7、14d抽血检测血清的ALT和AST.术后2wk用免疫组织化学方法检测各组肿瘤细胞Ⅷ因子及VEGF的表达情况,对Ⅷ因子染色阳性血管内皮细胞进行MVD计数.结果:介入治疗前1d,3组动物ALT、AST水平差异无显著性;介入治疗后3和7d,3组动物ALT和AST水平相比差异有统计学意义(F=28.411,55.537,均P<0.05;F=8.565,6.401,均P<0.05),C组与B组相比差异有统计学意义(P<0.05).3组动物MVD值和VEGF的表达强度以C组最低,B组最高,3组相比差异有统计学意义(F=5.22,P<0.05;χ2=7.247,P<0.05),C组与B组相比差异有统计学意义(P<0.05).结论:槐耳清膏可以改善动物TACE术后的肝功能,并可通过减少VEGF表达,抑制肿瘤血管生成达到治疗肝癌的目的.

AIM： To investigate the therapeutic effect of Huaier extract combined with transcatheterized arterial chemoembOlization on the expression of VEGF and MVD and hepatic function of rabbit VX2 hepatocarcinoma. METHODS： Thirty six rabbits with experimental liver cancer diagnosed by MRI were randomly divided into 3 groups. The rabbits in group A （control group）were infused with 0.2 mL/kg physiological saline via the hepatic artery, group B （TACE group） were infused with lipiodol 0.2 mL/kg＋MMC 0.5 mg/kg via the hepatic artery while group C（TACE ＋ administration with huaier extract ）were given by oral administration with 500mg/（kg·d） Huaier extract after TACE. The levels of AST and ALT in serum were respectively tested 1 day before operation, 3 days, 7 days and 14 days after operation. Two weeks after operation, the rabbits were sacrificed and the tumors were taken out. Immunohistochemical staining was performed to detect the expression and localization of factor Ⅶ and VEGF. The MVD was calculated by counting the factor Ⅷ positive endothelial cells. RESULTS： There was no of rabbits＇ serum AST and significant difference ALT among 3 groups 1 day before operation. However, 3 and 7 days after operation, there was significant difference of rabbits＇ serum AST and ALT among 3 groups （F = 28.411, 55.537, both P 〈 0.05; F = 8.565, 6.401, both P 〈 0.05）, and there was significant difference between group B and group C （P 〈 0.05）. The MVD and the positive express of VEGF were the lowest in group C, the highest in group B. There was significant difference among 3 groups （F = 5.22, P 〈 0.05; %2 =7.247, P 〈 0.05）, and there was significant difference between group B and group C （P 〈 0.05）. CONCLUSION： The Huaier extract can improve the hepatic function of rabbit VX2 hepatocarcinoma after TACE, which works on hepatocar- cinoma through diminishing the expression of VEGF and inhibiting the angiogenesis of tumor.