相溶解度法测定不同pH值下丹参酮Ⅱ_A-HP-β-CyD包合稳定常数

Effect of different pH on the inclusion stability constant of tanshinoneⅡ_A with hydroxylpropyl-β-cyclodextrin by phase solubility analysis

目的:利用相溶解度法研究不同pH值溶液下丹参酮ⅡA-HP-β-CyD包合稳定常数的变化。方法:用不同pH值的溶液配制一系列浓度的HP-β-CyD溶液,加入过量的丹参酮IIA提取物,绘制相溶解度图,并采用薄层色谱法对在包合物进行验证。结果:相溶解度曲线为线性关系,包合模型为AL型。当溶液呈酸性时,丹参酮ⅡA-HP-β-CyD包合物的包合稳定常数随着pH值的增大而逐渐减小,包合物越不稳定;当溶液呈碱性时,丹参酮ⅡA-HP-β-CyD包合物的包合稳定常数随着pH值的增大而逐渐增大。结论:结果表明HP-β-CyD对丹参酮IIA有很好的增溶效果,在常温和酸性条件下对丹参酮ⅡA-HP-β-CyD的包合有利。

AIM ： To study the effect of different pH medium on the inclusion stability constants of tanshinoneIIA with hydroxypropyl-β- cyclodextrin using phase solubility analysis. METHODS： Different concentrations of hydroxypropyl-β-cyclodextrin solution were preparated to determine tanshinone IIA content under the condition of overdose extract of tanshinone IIA and made a phase solubility diagram. The inclusion of tanshinonelIA with hydroxypropyl-β-cyclodextrin was verified by TLC. RESULTS： The relationship of phase solubility was approximatively linear and AL model of inclution compound. The inclusion stability constant of tanshinonelIA with hydroxylpropyl-β-cyclodextrin gradually decreased with a increase in pH and the inclution compound wasn＇t stable;The result was quite opposite to what solution was basic alkaline. CONCLUSION： HP-β-CyD could evidently increase the solubility of tanshinonelIA in water in the presence of acidification and room temperature.