参芍心欣方有效部位化学成分研究

Chemical constituents of Shenshao Xinxin Prescription

;目的:研究参芍心欣方(人参、赤芍、葛根)有效部位的化学成分,阐明其抗心功能不全的物质基础。方法:采用硅胶、聚酰胺、交联葡萄糖凝胶等多种柱层析手段,根据化合物性质和紫外、快中子轰击质谱核磁光谱数据鉴定结构。结果:从参芍心欣方有效部位的三个部分中分离得到8个单体化合物,它们分别为:大豆素、4′-甲氧基大豆苷、染料木苷、3′-甲氧基葛根素、葛根素、芍药苷、葛根素芹菜糖苷、大豆苷元4′、7-葡萄糖苷。结论:分离得到的化合物能部分反映出该复方醇溶性的有效部位。

AIM： To investigate the constituents of the effective fractions from Shenshao Xinxin Prescription （Radix et rhizoma ginseng; Radix paeoniae rubra; Radix puerariae lobatae）. METHODS： The compounds were isolated by column chromatography on silica gel, polyamide, sephadex gel and their structures were determined on the basis of their spectroscopic evidence including UV, FAB-MS and NMR data. RESULTS： Eight compounds had been isolated and identified as daidzein, 4＇-O-methyl-daidzin, genistin, PG-3, puerarin, paeoniflorin, daidzein 8-C-Apiosyl （1→6） glucoside, 4＇ ,7-diglucoside. CONCLUSION： The compounds obtained were the alcohol-soluble compounds from Shenshao Xinxin Prescription.