摘要
蛋黄中含有大量磷脂,其中磷脂酰胆碱(PC)和磷脂酰乙醇胺(PE)最为丰富。本研究采用高效液相色谱法(HPLC)与基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)联用技术分析了蛋黄中磷脂粗提物。将从蛋黄中提取的多种磷脂通过HPLC预先分离,收集各组分后分别进行MALDI-TOF MS分析得到比较清晰的质谱图。通过质谱图解析确定了蛋黄中磷脂酰胆碱、神经鞘磷脂(SM)的脂肪酸组成。
Egg yolk contains a variety of different phospholipids, of which phosphatidylcholine （PC） and phosphati- dylethanolamine （PE） are the most abundant molecular classes. A method of offline combining MALDI-TOF MS and HPLC for the analysis of Pl_s is reported here. Total PLs of hen egg yolk are extracted and then separated by HPLC be-fore the obtained subfractions are analyzed by MALDI-TOF MS. With this procedure of offline HPLC-MALDI-TOF- MS, the well-resolved mass spectra of individual lipid classes are successfully obtained. The composition analysis of PC and sphingomyelin （SM） species in egg yolk are given by resolving the spectra obtained.
出处
《分析试验室》
CAS
CSCD
北大核心
2009年第4期30-33,共4页
Chinese Journal of Analysis Laboratory
基金
中国科学院知识创新工程重要方向(KSCX2-YW-N-035)
沈阳市科技计划2007GX-49项目资助
