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乳源酪蛋白糖巨肽分离条件的研究 被引量:2

Isolation of κ-Casein Glycomacropeptide(CGMP)Derived from Milk
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摘要 探讨了利用(NH4)2SO4分级沉淀、三氯乙酸(TCA)纯化酪蛋白的胃蛋白酶水解物中酪蛋白糖巨肽(CGMP)的工艺。结果表明:经过20%和65%饱和度的(NH4)2SO4沉淀胃蛋白酶水解物、12%TCA纯化得到的样品,其纯度为91.2%。整个工艺路线简便、快捷、成本较低,适合工业化生产。 The conditions of isolating and purifying casein glycomacropeptide (CGMP) were studied by ammonium sulfate precipitation and TCA. The results show that the purity of CGMP produced was 91.2% when 20% and 65% ammonium sulfate precipitation and 12% TCA were used. The entire processing conditions were simple, fast, low cost, so it was suitable for the industry.
出处 《天津农学院学报》 CAS 2009年第1期32-34,共3页 Journal of Tianjin Agricultural University
基金 天津农学院科学研究发展基金项目"乳源酪蛋白糖巨肽的分离纯化研究"(重点2007002)
关键词 酪蛋白糖巨肽(CGMP) 胃蛋白酶 盐析 唾液酸 casein glycomacropeptide (CGMP) pepsin salting-out: sialicacid
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  • 1董安玮.史氏始叶螨生物学特性及其防治初步研究[J].昆虫天敌,1993,15(2):73-77. 被引量:1
  • 2彭增起.牛乳中糖蛋白的保健功能[J].中国乳品工业,1996,24(1):22-23. 被引量:6
  • 3张智武,张列兵,白淑贤.酪蛋白巨肽的特性及应用前景[J].中国乳品工业,1997,25(2):39-41. 被引量:7
  • 4Saito T, Itoh T, Yamaji A. A new isolation method of caseinoglycopiptide from sweet cheese whey. Journal of Dairy Science, 1991, 74:2831-2837.
  • 5Eigel W N, Butler J E, Ernstrom C A, Whitney RMcL.Nomenclature of proteins of cow's milk. Journai of Dairy Science, 1984,67:1599-1631.
  • 6Skudder P J. Evaluation of a porous silica-based ion-exchange medium for the production of protein fractions from rennet-and acid whey. Journal of Dairy Research, 1985, 52:167-181.
  • 7Fiat A-M, Jolles P. Caseins of various origins and biologically active casein peptides and oligosaccharides:structural and physiological aspects. Molecular and Cellular Biochemistry, 1989, 87:5-30.
  • 8Jolles J, Fiat A-M. Comparative study of cow and sheep κ - caseino glycopeptides and location of the srgars. REBS Letters, 1995, 30:173-176.
  • 9Otani H, Monnai M, Kawasaki Y, Tanimoto M.Inhibition of mitogen-induced proliferative responses of lymphocytes by bovine K - caseino glycopeptides having different carbohydrate chains. Journal of Dairy Research, 1995, 62:349-357.
  • 10Fiat A-M, Jolles J,et al. Localization of the prosthetic sugar guoups of bovine colostrums κ -casein.Hoppe-Seyler's Zeitschrift fur Physiologische Chemie,1981, 362:1447-1454.

共引文献31

同被引文献22

  • 1李武祎,郭顺堂,俞树孝,陆晓民.酪蛋白组分分离技术及消化性测定[J].食品科学,2005,26(7):70-73. 被引量:12
  • 2FanaroS,VigiV.Protein quality and quantity in infant formulas.A critical look[J].Minerva Pediatr,2002,54(3):203-209.
  • 3RojasE,TorresG.Isolation and recovery of glycomacropeptide from milk whey by means of thermal treatment[J].Food Sci Technol,2013,33(1):14-20.
  • 4Neelima,SharmaR,RajputYS,et al.Chemical and functional properties of glycomacropeptide (GMP) and its role in the detection of cheese whey adulteration in milk:a review[J].Dairy Sci Technol,2013,93(1):21-43.
  • 5NakanoT,OzimekL.A sialic acid assay in isolation and purification of bovine k-casein glycomacropeptide:a review[J].Recent Pat Food Nutr Agric,2014,6(1):38-44.
  • 6WangB,Brand-MillerJ,McveaghP,et al.Concentration and distribution of sialic acid in human milk and infant formulas[J].Am J Clin Nutr,2001,74(4):510-515.
  • 7CristofaloEA,SchanlerRJ,BlancoCL,et al.Randomized trial of exclusive human milk versus preterm formula diets in extremely premature infants[J].J Pediatr,2013,163(6):1592-1595.
  • 8Thom?-WorringerC,S?rensenJ,López-Fandi?oR.Health effects and technological features of caseinomacropeptide[J].Int Dairy J,2006,16(11):1324-1333.
  • 9徐静波,孟祥河,叶欧,缪莉,孙培龙,张建友.乳清中酪蛋白糖巨肽的制备及纯化研究[J].河南工业大学学报(自然科学版),2008,29(1):56-59. 被引量:2
  • 10张秀媛,袁永俊.凝乳酶水解酪蛋白生产酪蛋白糖巨肽工艺条件的研究[J].食品工业科技,2008,29(4):171-173. 被引量:8

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