摘要
探讨了利用(NH4)2SO4分级沉淀、三氯乙酸(TCA)纯化酪蛋白的胃蛋白酶水解物中酪蛋白糖巨肽(CGMP)的工艺。结果表明:经过20%和65%饱和度的(NH4)2SO4沉淀胃蛋白酶水解物、12%TCA纯化得到的样品,其纯度为91.2%。整个工艺路线简便、快捷、成本较低,适合工业化生产。
The conditions of isolating and purifying casein glycomacropeptide (CGMP) were studied by ammonium sulfate precipitation and TCA. The results show that the purity of CGMP produced was 91.2% when 20% and 65% ammonium sulfate precipitation and 12% TCA were used. The entire processing conditions were simple, fast, low cost, so it was suitable for the industry.
出处
《天津农学院学报》
CAS
2009年第1期32-34,共3页
Journal of Tianjin Agricultural University
基金
天津农学院科学研究发展基金项目"乳源酪蛋白糖巨肽的分离纯化研究"(重点2007002)