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MSTN RNAi双元表达载体对成肌细胞增殖及分化的影响 被引量：3

Effects of MSTN RNAi Binary Expression Vector on Myoblast Hyperplasia and Differentiation
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摘要 为了确定MSTN RNAi双元表达载体pEGFP-N1-M1-M2对成肌细胞增殖和分化的影响,利用脂质体介导法将该双元表达载体转染成肌细胞,MTT法检测MSTN RNAi双元表达载体对成肌细胞增殖的作用,Giemsa染色检测pEGFP-N1-M1-M2双元表达载体对成肌细胞融合率的影响。试验结果表明,pEGFP-N1-M1-M2双元表达载体有促进成肌细胞增殖和分化的作用,能够促进成肌细胞大量融合。该结果为进一步探讨MSTN基因的作用机理提供了更为便捷的研究方法。 To decide the effect of pEGFP-N1-M1-M2 binary vector to myoblast cell, the binary vector was transfected into myoblast ceils by lipidosome, and myoblast hyperplasia and differentiation was detected by MTT and Giemsa assay. The results showed that pEGFP-N1-M1-M2 binary vector could promote myoblast hyperplasia, differentiation and fusion.The results provided easier experimental way to study the mechanism of MSTN gene.
作者 吴丹 胡兰
机构地区 沈阳农业大学畜牧兽医学院
出处 《沈阳农业大学学报》 CAS CSCD 北大核心 2009年第1期38-42,共5页 Journal of Shenyang Agricultural University
基金 辽宁省自然科学基金资助项目(20050302) 辽宁省教育厅科研基金资助项目(2007304)
关键词 MSTN基因 双元表达载体 成肌细胞 增殖分化 MSTN gene binary expression vector myoblast hyperplasia and differentiation
分类号 S831 [农业科学—畜牧学] S813.3 [农业科学—畜牧学]
  • 相关文献

参考文献10

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二级参考文献6

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同被引文献43

  • 1胡兰,王娜,胡锐,刘梅,石娇.大骨鸡MSTN基因的表达检测[J].中国家禽,2003,25(z1):46-48. 被引量：13
  • 2杨晓静,陈杰,胥清富,韦习会,赵茹茜.二花脸猪和大白猪背最长肌中肌肉生长抑制素和生肌调节因子基因的表达及其性别特点[J].南京农业大学学报,2006,29(3):64-68. 被引量：15
  • 3柳玲.相对定量的2^(-△△CT)法分析HLA-G基因在子宫内膜异位症中的表达[J].中国优生与遗传杂志,2007,15(9):25-26. 被引量：16
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二级引证文献10

沈阳农业大学学报
2009年 第1期

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