日本血吸虫Sj28GST重组抗原的摇瓶发酵条件研究

Optimal condition of expression of Sj28GST in the recombinant E.coli TB1 with shaking flask fermentation

目的探索应用重组E.coliTB1摇瓶生产日本血吸虫Sj28GST重组抗原的最适工艺条件。方法用2YT培养基进行发酵,观察培养温度、种子液接种量、诱导剂异丙基-β-D硫代半乳糖苷(IPTG)浓度、IPTG诱导时间等培养条件。结果培养温度为37℃,种子液接种量为10%,转速为200 r/m in,用0.8 mmol/L IPTG在开始发酵4 h后进行诱导,为最佳发酵条件。在此条件下,振荡培养17 h后,细菌密度达到吸光度(A600)值2.5,重组日本血吸虫Sj28GST产量达到21.6mg/L发酵液。结论成功探索了日本血吸虫Sj28GST重组抗原的摇瓶发酵条件。

Objective To explore the production conditions of recombinant Sj28GST in E.coli TB1 with shaking flask fermentation.Methods The culture temperature,amount of inoculums,IPTG concentration and the initial induction time were explored.Results The optimal expression conditions of Sj28GST in E.coli TB1 were culture temperature being 37 ℃,amount of inoculums being 10%,IPTG concentration being 0.8 mmol/L,rotational speed being 200 r/min,initial induction time being 4 h after the culture beginning.Under this condition,the bacterial density（A600） was about 2.5 and the production of Sj28GST reached 21.6 mg/L.Conclusion It is successful to explore the production conditions of recombinant Sj28GST with shaking flask fermentation.