白藜芦醇对高糖高脂处理的原代人脐静脉血管内皮细胞E-选择素表达及NO分泌的影响

Effects of resveratrol on the expression of E-selectin and secretion of nitric oxide of primary cultured human umbilical vein endothelial cells with high glucose and lipid

目的:探讨白藜芦醇对高糖、高脂培养人原代脐静脉血管内皮细胞E-选择素mRNA表达及胰岛素刺激的一氧化氮(NO)分泌的影响。方法:原代培养人脐静脉内皮细胞,以0.1μmol/L白藜芦醇预处理4h后,以高糖(33.3mmol/L葡萄糖)+高脂(棕榈酸0.5mmol/L)DMEM培养基培养24h,以RT-PCR法检测培养细胞E-选择素的表达,部分细胞经100nmol/L胰岛素刺激25min后,以硝酸还原酶法检测培养上清NO的含量。结果:与正常对照组相比,高糖高脂培养使内皮细胞E-选择素的表达升高,同时胰岛素刺激的NO分泌降低。而白藜芦醇预处理可以明显降低E-选择素的表达,并明显促进胰岛素对内皮细胞NO分泌的刺激作用。结论:白藜芦醇可以改善高糖、高脂诱导的血管内皮细胞胰岛素刺激的NO分泌作用,并可降低E-选择素表达,保护内皮细胞的功能,从而可能在糖尿病动脉粥样硬化的防治中具有广阔的前景。

Objective： To investigate the effects of resveratrol on the expression of E-selectin and nitric oxide （NO） secretion induced by insulin in cultured primary human umbilical vein endothelial cells （HUVEC） with high glucose and palmitate acid. Methods： Primarily cultured HUVEC was pretreated with resveratrol（0.1 μmol/L） for 4 hours, then cultured in DMEM with 0.5 mmol/L palmitate acid and 33.3 mmol/L glucose for 24 hours. The cells were collected to analyze the expression of E-selectin. Supernatant of cultured cells,induced by 100 nmol/L insulin for 25 min, was used to analyze the NO content. Results： Compared with normal control cells, the secretion of NO stimulated by insulin decreased, but the expression of E-selectin increased in HUVEC. Resveratrol treatment increased the secretion of NO stimulated by insulin and decreased the expression of E-selectin, partly counteract the impairment of high glucose and palmitate acid on the function of endothelial cells. Conclusion： Resveratrol can improve and protect the function of high glucose and fatty acid cultured endothelial cell, and therefore may be a promise medicine in the prevention or therapy of diabetic macrovascular diseases.