不同处理方式对奶牛粪细菌群落多样性及群落结构的影响

Effects of Different Treat Ways on Microbial Diversity and Microbial Community Structure of Dairy Manure

以腐熟牛粪、新鲜牛粪以及蚯蚓粪为材料,提取其微生物总DNA,利用细菌16SrDNAV3区扩增及变性梯度凝胶电泳(PCR-DGGE)法,分析了3种材料中细菌的多样性以及细菌群落结构的相似性。结果表明,蚯蚓粪的细菌多样性最丰富,腐熟牛粪次之,新鲜牛粪的细菌多样性最小。蚯蚓粪和腐熟牛粪的细菌群落结构有40%的相似性;蚯蚓粪和新鲜牛粪的细菌群落结构的相似性为25%;新鲜牛粪和腐熟牛粪的细菌群落结构的相似性则为35%。

Effects of different treat ways on microbial diversity and microbial community structure of dairy manure were studied by PCRDGGE profiles of bacterial 16S rDNA V3 region. In this research, composted dairy manure and vermicompost came from fresh dairy manure by composting and vermicomposting. The total genomic DNA of composted dairy manure, fresh dairy manure and vermicompost were extracted respectively by direct DNA extraction method. By analyzing the DGGE profiles, it was found that the microbial diversity of vermicompost was higher than that of fresh dairy manure, but lower than that of composted dairy manure. There were about 40% similarities of microbial community structure between vermieompost and composted dairy manure, 25% between vermieompost and fresh dairy manure, and 35% between fresh dairy manure and composted dairy manure. The research result indicates that different treat ways will result in the distinct change of microbial diversity and microbial community structure of dairy manure, moreover, the change arises from vermicomposting is more significant than that from composting. This study will help us to know the roles of microorganisms in the process of composting or vennicomposting of dairy manure.