δ阿片受体激动剂DADLE对全脑缺血再灌注大鼠大脑组织病理影响

Effect of the δ-opioid Receptor Agonist DADLE on Pathologic Changes in the Cerebral Tissue after Global Cerebral Ischemia and Reperfusion in Rats

目的:观察δ阿片受体激动剂DADLE对全脑缺血再灌注大鼠大脑组织结构病理变化的影响,探讨DADLE在脑复苏中的神经保护作用。方法:50只SD大鼠随机分为5组,每组各10只:①假手术组(N):不制模不干预;②模型组(I/R):单纯制作全脑缺血再灌注模型;③DADLE预处理组(D1):在缺血前给予DADLE;④DADLE缺血后处理组(D2):在缺血后给予DADLE;⑤DADLE再灌注期处理组(D3):在再灌注早期给予DADLE。实验结束后,取大脑组织进行形态学检查。结果:全脑缺血再灌注大鼠大脑出现大量神经元坏死、胞浆浓缩、核固缩现象。DADLE各处理组缺血再灌注损伤的病理改变明显减轻。模型组和DADLE各处理组海马神经元细胞密度均低于假手术组(P<0.01),而DADLE各处理组海马神经元细胞密度高于模型组(P<0.01),DADLE各处理组之间海马神经元细胞密度差异没有统计学意义(P>0.05)。结论:δ阿片受体激动剂DADLE对大鼠全脑缺血再灌注损伤具有一定的保护作用。

Objective： To observe influence of theδ-opioid receptor DADLE on global cerebral ischemia and reperfusion in rats with the pathologic changes in the cerebral tissue and explore the neuroproteetive effect of DADLE in cerebral resuscitation. Methods： Fifty SD rats were randomly divided into five groups： sham operation group, model group, DADLE pretreatment group, DADLE post-treatment group and DADLE treatment during reperfusion group （n=10 for each group）. ①Sham operational group was operated without isehemia and treatment; ②the model group was rendered global cerebral ischemia and reperfusion model but received no intervention; ③DADLE pretreatment group received DADLE treatment before ischemia; ④DADLE post-treatment group received DADLE treatment immediately after ischemia;⑤DADLE treatment during reperfusion group received DADLE treatment during early reperfusion. The five groups were collected for cerebral tissue to be detected for pathological change. Results： The model group exhibited obvious pathological lesion of nerve cells including neuronal necrosis, concentrated neuronal cytoplasma and karyopycnosis, compared with the milder changes of neurons in all DADLE- treated groups. The neuron density in hippocampus area was lower in the model group and each DADLE-treated group than as seen for in the sham operation group（P〈0.01）. Compared with model group, the neuron density in hippocampus of each DADLE-treated group area was higher （P〈0.01）. There were no differences in the neuron density in hippocampus area among all DADLE-treated groups （P〉0.05）. Conclusion： The δ-opioid receptor DADLE may be neuroprotective against global cerebral ischemia and reperfusion in rats.