三七丹参对实验性肝纤维化的影响

Effect of Sanqidanshen Tablet on experimental liver fibrosis

目的:探讨不同剂量三七丹参(Sanqidanshen Tablet)对四氯化碳(CCl4)所致实验性肝纤维化的防治作用及量效关系。方法:采用CCl4复制小鼠肝纤维化模型,同时用0.5g/kg、1g/kg和2g/kg的三七丹参治疗,测定血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、白蛋白(ALB)、透明质酸(HA),Ⅲ型前胶原(PCⅢ),IV型胶原(IV-C),层粘蛋白(LN),并以光镜观察肝脏组织学改变,进行炎症活动度分级及肝纤维化分期。综合分析不同剂量三七丹参对CCl4中毒性肝纤维化的防治作用。结果:高、中、低剂量三七丹参均有降低ALT、AST的作用,并显著降低HA、PCⅢ和IVC。经治疗后的肝细胞变性、坏死及纤维组织增生明显减少。其中高、中剂量组的疗效比低剂量组好,而高、中剂量组两者之间的差异无显著性。结论:表明三七丹参具有抗实验性肝纤维化的作用,三七丹参高、中剂量组防治肝纤维化的作用优于低剂量组。

Objective：To investigate the effect of the various doses treatment groups of Sanqidanshen Tablet on liver fibrosis （LF） induced by CCl4. Methods：The treatment groups of Sanqidanshen Tablet were divided into high dose group （2 g/kg）, medium dose group （1 g/kg）and low dose group （0.5 g/kg）. Serum levels of ALT,AST,ALB,HA,LN,PC Ⅲ ,and Ⅳ-C were determined. The pathological changes of hepatic tissues and grade of liver inflammation and stage of liver fibrosis （GS） were analyzed. Results：Serum levels of ALT, AST,HA,LN,PC III and Ⅳ-C in the treatment groups were obviously lower than those in the model group,and the pathological results under microscope and degree of GS were much better as compared with the model group. The effect of high dose group was similar to the medium dose group and these two groups were much better than the low dose group. Conclusion：Sanqidansheta Tablet shows preventive effect against LF induced by CCl4 and the action of high and medium dose groups is stronger than the low dose group.